publication . Article . 2012

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

Corman, V. M.; Eckerle, I.; Bleicker, T.; Zaki, A.; Landt, O.; Eschbach-Bludau, M.; Van Boheemen, S.; Gopal, R.; Ballhause, M.; Bestebroer, T. M.; ...
Open Access English
  • Published: 27 Sep 2012
  • Publisher: EUR CENTRE DIS PREVENTION & CONTROL
  • Country: Netherlands
Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
Persistent Identifiers
Subjects
Medical Subject Headings: virusesvirus diseases
free text keywords: Virology, Public Health, Environmental and Occupational Health, Epidemiology, Virology, Biology, Middle East respiratory syndrome coronavirus, medicine.disease_cause, medicine, Polymerase chain reaction, law.invention, law, Respiratory virus, Viral replication, Real-time polymerase chain reaction, Reverse transcription polymerase chain reaction, Molecular biology, Coronavirus, Open reading frame
Communities
  • COVID-19
Funded by
EC| EMPERIE
Project
EMPERIE
European Management Platform for Emerging and Re-emerging Infectious disease Entities
  • Funder: European Commission (EC)
  • Project Code: 223498
  • Funding stream: FP7 | SP1 | HEALTH
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