publication . Article . 2021

SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance

Robinot, Rémy; Hubert, Mathieu; de Melo, Guilherme Dias; Lazarini, Françoise; Bruel, Timothée; Smith, Nikaïa; Levallois, Sylvain; Larrous, Florence; Fernandes, Julien; Gellenoncourt, Stacy; ...
Open Access
  • Published: 16 Jul 2021 Journal: Nature Communications, volume 12 (eissn: 2041-1723, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
  • Country: France
Abstract
Understanding how SARS-CoV-2 spreads within the respiratory tract is important to define the parameters controlling the severity of COVID-19. Here we examine the functional and structural consequences of SARS-CoV-2 infection in a reconstructed human bronchial epithelium model. SARS-CoV-2 replication causes a transient decrease in epithelial barrier function and disruption of tight junctions, though viral particle crossing remains limited. Rather, SARS-CoV-2 replication leads to a rapid loss of the ciliary layer, characterized at the ultrastructural level by axoneme loss and misorientation of remaining basal bodies. Downregulation of the master regulator of cilio...
Persistent Identifiers
Subjects
Medical Subject Headings: respiratory systemrespiratory tract diseasesskin and connective tissue diseasesfungiviruses
free text keywords: General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, MESH: Animals, MESH: Axoneme, MESH: Male, MESH: Mesocricetus, MESH: Mucociliary Clearance, MESH: Respiratory Mucosa, MESH: SARS-CoV-2, MESH: Virus Replication, MESH: Basal Bodies, MESH: COVID-19, MESH: Cilia, MESH: Cricetinae, MESH: Cytokines, MESH: Epithelial Cells, MESH: Forkhead Transcription Factors, MESH: Humans, MESH: Lung, [SDV.IMM]Life Sciences [q-bio]/Immunology, Article, Cilia, SARS-CoV-2, Science, Q
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