publication . Conference object . Other literature type . 2020

BS5.3 - Piezoelectric quartz crystal aptamer biosensor for detection and quantification of SARS CoV helicase protein

D. R. B. Albano; K. Shum; J. A. Tanner; Y. S. Fung;
Open Access
  • Published: 18 Dec 2020
  • Publisher: AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany
Abstract
To meet the highly demanding challenge for detecting protein biomarkers at pg/mL level in highprotein sera sample, the coupling of paramagnetic nanoparticle technology with aptamer-PQC biosensor was attempted for developing aptamer-PQC biosensor for selective detection of SCV helicase protein produced from SARS CoV replication. The coupling of aptamer coated paramagnetic nanoparticles for sample pretreatment to aptamer-PQC biosensor has shown to detect helicase protein in one-minute assay with a detection limit of 3.5 ng/mL. The aptamer-coated crystal exhibits a frequency shift linearly proportional to the concentration of SARS helicase from 0.05 to 1 μg/mL with...
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free text keywords: PQC, SARS, SCV helicase, aptamer, flow injection analysis, magnetic bead, Materials science, Biosensor, Aptamer, Helicase, biology.protein, biology, Biophysics, Piezoelectric quartz
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COVID-19
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