publication . Article . 2017

Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection

Xingdong Zhou; Yingying Cong; Tineke Veenendaal; Judith Klumperman; Dongfang Shi; Muriel Mari; Fulvio Reggiori;
Open Access English
  • Published: 05 Sep 2017
  • Country: Netherlands
Abstract
The porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) belonging to the alpha-CoV genus and it causes high mortality in infected sucking piglets, resulting in substantial losses in the farming industry. CoV trigger a drastic reorganization of host cell membranes to promote their replication and egression, but a detailed description of the intracellular remodeling induced by PEDV is still missing. In this study, we examined qualitatively and quantitatively, using electron microscopy, the intracellular membrane reorganization induced by PEDV over the course of an infection. With our ultrastructural approach, we reveal that, as most of CoV, PEDV initially forms double-membrane vesicles (DMVs) and convoluted membranes (CMs), which probably serve as replication/transcription platforms. Interestingly, we also found that viral particles start to form almost simultaneously in both the endoplasmic reticulum and the large virion-containing vacuoles (LVCVs), which are compartments originating from the Golgi, confirming that alpha-CoV assemble indistinguishably in two different organelles of the secretory pathway. Moreover, PEDV virons appear to have an immature and a mature form, similar to another alpha-CoV the transmissible gastroenteritis coronavirus (TGEV). Altogether, our study underlies the similarities and differences between the lifecycle of alpha-CoV and that of viruses belonging to other CoV subfamilies.
Fields of Science and Technology classification (FOS)
03 medical and health sciences, 0301 basic medicine, 030104 developmental biology, 030106 microbiology
Subjects
Medical Subject Headings: virusesvirus diseasesrespiratory tract diseases
ACM Computing Classification System: ComputingMethodologies_DOCUMENTANDTEXTPROCESSINGGeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
free text keywords: PEDV, alpha-coronavirus, lifecycle, electron microscopy, membrane rearrangement, CORONAVIRUS NUCLEOCAPSID PROTEIN, SARS-CORONAVIRUS, ENDOPLASMIC-RETICULUM, MOUSE HEPATITIS, CELL-CULTURE, REPLICATION, MORPHOGENESIS, PROLIFERATION, PATHOGENESIS, INHIBITION, Virology, Infectious Diseases, Journal Article, Article, PEDV; <i>alpha-coronavirus</i>; lifecycle; electron microscopy; membrane rearrangement, Animals, Chlorocebus aethiops, Endoplasmic Reticulum/metabolism, Intracellular Membranes/metabolism, Microscopy, Electron, Porcine epidemic diarrhea virus/isolation & purification, Swine, Vacuoles/ultrastructure, Vero Cells, Porcine epidemic diarrhea virus, biology.organism_classification, biology, Endoplasmic reticulum, Coronavirus, medicine.disease_cause, medicine, Vacuole, Golgi apparatus, symbols.namesake, symbols, Intracellular, Secretory pathway, Virology, Microbiology, Transmissible gastroenteritis coronavirus, lcsh:Microbiology, lcsh:QR1-502
Communities
  • COVID-19
Funded by
NWO| A three-dimensional look into autophagy
Project
  • Funder: Netherlands Organisation for Scientific Research (NWO) (NWO)
  • Project Code: 2300175771
,
EC| PRONKJEWAIL
Project
PRONKJEWAIL
Protecting patients with enhanced susceptibility to infections
  • Funder: European Commission (EC)
  • Project Code: 713660
  • Funding stream: H2020 | MSCA-COFUND-DP
Open Access
NARCIS
Article . 2017
Providers: NARCIS
Open Access
Viruses
Article
Providers: UnpayWall
Open Access
Viruses
Article . 2017
Providers: PubMed Central
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