publication . 2021

Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells

Bram M. ter Ellen; Nilima Dinesh Kumar; Ellen M Bouma; Berit Troost; Denise P. I. van de Pol; Heidi van der Ende-Metselaar; Leonie Apperloo; Djoke van Gosliga; Maarten van den Berge; Martijn C. Nawijn; ...
Open Access
  • Published: 10 Jul 2021 Journal: Viruses, volume 13, page 1,335 (eissn: 1999-4915, Copyright policy)
  • Publisher: MDPI AG
The current COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has an enormous impact on human health and economy. In search for therapeutic options, researchers have proposed resveratrol, a food supplement with known antiviral, anti-inflammatory, and antioxidant properties as an advantageous antiviral therapy for SARS-CoV-2 infection. Here, we provide evidence that both resveratrol and its metabolically more stable structural analog, pterostilbene, exhibit potent antiviral properties against SARS-CoV-2 in vitro. First, we show that resveratrol and pterostilbene antiviral activity in African green monkey kidney cells. Both compounds actively inhibit virus replication within infected cells as reduced virus progeny production was observed when the compound was added at post-inoculation conditions. Without replenishment of the compound, antiviral activity was observed up to roughly five rounds of replication, demonstrating the long-lasting effect of these compounds. Second, as the upper respiratory tract represents the initial site of SARS-CoV-2 replication, we also assessed antiviral activity in air–liquid interface (ALI) cultured human primary bronchial epithelial cells, isolated from healthy volunteers. Resveratrol and pterostilbene showed a strong antiviral effect in these cells up to 48 h post-infection. Collectively, our data indicate that resveratrol and pterostilbene are promising antiviral compounds to inhibit SARS-CoV-2 infection. Because these results represent laboratory findings in cells, we advocate evaluation of these compounds in clinical trials before statements are made whether these drugs are advantageous for COVID-19 treatment.
Persistent Identifiers
Fields of Science and Technology classification (FOS)
03 medical and health sciences, 0301 basic medicine, 0302 clinical medicine, 030220 oncology & carcinogenesis, 030304 developmental biology
Sustainable Development Goals (SDG)
3. Good health
free text keywords: Virology, Infectious Diseases, Pharmacology, Pterostilbene, chemistry.chemical_compound, chemistry, In vitro, Cell culture, Respiratory tract, medicine.anatomical_structure, medicine, Vero cell, Virus, Resveratrol, Viral replication
Related Organizations
Funded by
Protecting patients with enhanced susceptibility to infections
  • Funder: European Commission (EC)
  • Project Code: 713660
  • Funding stream: H2020 | MSCA-COFUND-DP
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