publication . Article . 2017

Diagnostic Accuracy of FebriDx: A Rapid Test to Detect Immune Responses to Viral and Bacterial Upper Respiratory Infections

Wesley H. Self; Jeffrey Rosen; Stephan C Sharp; Michael R. Filbin; Peter C. Hou; Amisha Parekh; Michael C. Kurz; Nathan I. Shapiro;
Open Access
  • Published: 07 Oct 2017
  • Publisher: MDPI AG
Abstract
C-reactive protein (CRP) and myxovirus resistance protein A (MxA) are associated with bacterial and viral infections, respectively. We conducted a prospective, multicenter, cross-sectional study of adults and children with febrile upper respiratory tract infections (URIs) to evaluate the diagnostic accuracy of a rapid CRP/MxA immunoassay to identify clinically significant bacterial infection with host response and acute pathogenic viral infection. The reference standard for classifying URI etiology was an algorithm that included throat bacterial culture, upper respiratory PCR for viral and atypical pathogens, procalcitonin, white blood cell count, and bandemia. ...
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free text keywords: Article, respiratory infection, antibiotic stewardship, MxA, CRP, immunoassay, FebriDx, Respiratory infection, Medicine, business.industry, business, Internal medicine, medicine.medical_specialty, Etiology, Respiratory tract infections, Procalcitonin, White blood cell, medicine.anatomical_structure, Microbiological culture, Bandemia, Immunology, Immune system, Gastroenterology, lcsh:Medicine, lcsh:R
23 references, page 1 of 2

Frequent detection of respiratory viruses without symptoms: Toward defining clinically relevant cutoff values. J. Clin. Microbiol. 2011, 49, 2631-2636. [CrossRef] [PubMed] 15. Jartti, T.; Jartti, L.; Peltola, V.; Maris, M.; Ruuskanen, O. Identification of respiratory viruses in asymptomatic subjects: Asymptomatic respiratory viral infections. Pediatr. Infect. Dis. J. 2008, 27, 1103-1107. [CrossRef] [PubMed] 16. FilmArray Respiratory Panel. BioMerieux. Available online: http://www.biomerieux-diagnostics.com/ filmarrayr-respiratory-panel (accessed on 29 April 2016). 17. EBV-VCA IgM Enzyme Immunoassay Test Kit. Diamedix. Available online: http://diamedix.com/wpcontent/uploads/2015/10/PI-EBVVCAIgM-720610-Rev7-June-15.pdf (accessed on 29 April 2016). 18. BRAHMS PCT Sensitive KRYPTOR. Thermo Fisher Scientific. Available online: http://www.procalcitonin.

com/ (accessed on 29 April 2016). 19. Korppi, M.; Kroger, L.; Laitinen, M. White blood cell and differential counts in acute respiratory viral and bacterial infections in children. Scand. J. Infect. Dis. 1993, 25, 435-440. [CrossRef] [PubMed] 20. Casey, J.R.; Marsocci, S.M.; Murphy, M.L.; Francis, A.B.; Pichichero, M.E. White blood cell count can aid judicious antibiotic prescribing in acute upper respiratory infections in children. Clin. Pediatr. 2003, 42, 113-119. [CrossRef] [PubMed] 21. Burkhardt, O.; Ewig, S.; Haagen, U.; Giersdorf, S.; Hartmann, O.; Wegscheider, K.; Hummers-Pradier, E.; Welte, T. Procalcitonin guidance and reduction of antibiotic use in acute respiratory tract infection.

29. Stewart, E.H.; Davis, B.; Clemans-Taylor, B.L.; Littenberg, B.; Estrada, C.A.; Centor, R.M. Rapid antigen Group A Streptococcus Test to diagnose pharyngitis: A systematic review and meta-analysis. PLoS ONE 2014, 9, e111727. [CrossRef] [PubMed] 30. Dingle, T.C.; Abbott, A.N.; Fang, F.C. Reflexive culture in adolescents and adults with Group A Streptococcal pharyngitis. Clin. Infect. Dis. 2014, 59, 643-650. [CrossRef] [PubMed] 31. Inverness Medical: BinaxNow Influenza A&B CLIA Waived (Package Insert Training Packet). Available online: http://www.amms.net/images/healthcare_industry_clia_binax_now_influenza_test_2009.pdf (accessed on 02 June 2016). 32. Uyeki, T.M.; Prasad, R.; Vukotich, C.; Stebbins, S.; Rinaldo, C.; Ferng, Y.H.; Morse, S.S.; Larson, E.L.; Aiello, A.E.; Davis, B.; et al. Low sensitivity of rapid diagnostics tests for influenza. Clin. Infect. Dis. 2009, 48, e89-e92. [CrossRef] [PubMed] 33. Self, W.H.; McNaughton, C.D.; Grijalva, C.G.; Zhu, Y.; Chappell, J.D.; Williams, J.V.; Talbot, H.K.; Shay, D.K.; Griffin, M.R. Diagnostic performance of the BinaxNow Influenza A&B rapid antigen test in ED patients.

Am. J. Emerg. Med. 2012, 30, 1955-1961. [PubMed] 34. Andreola, B.; Bressan, S.; Callegaro, S.; Liverani, A.; Plebani, M.; Da Dalt, L. Procalcitonin and C-reactive protein as diagnostic markers of severe bacterial infections in febrile infants and children in the emergency department. Pediatr. Infect. Dis. J. 2007, 26, 672-677. [CrossRef] [PubMed] 35. Bafadhel, M.; Clark, T.W.; Reid, C.; Medina, M.J.; Batham, S.; Barer, M.R.; Nicholson, K.G.; Brightling, C.E.

Procalcitonin and c-reactive protein in hospitalized adult patients with community-acquired pneumonia or exacerbation of asthma or COPD. Chest 2011, 139, 1410-1418. [CrossRef] [PubMed] 36. Sambursky, R.; Shapiro, N.I. Evaluation of a combined MxA and CRP point-of-care immunoassay to identify viral and/or bacterial immune response in patients with acute febrile respiratory infection. Eur. Clin. Respir. J.

2015, 2, 28245. [CrossRef] [PubMed] 37. Putto, A.; Ruuskanen, O.; Meurman, O.; Ekblad, H.; Korvenranta, H.; Mertsola, J.; Peltola, H.; Sarkkinen, H.; Viljanen, M.K.; Halonen, P. C reactive protein in the evaluation of febrile illness. Arch. Dis. Child. 1986, 61, 24-29. [CrossRef] [PubMed] 38. Hatherill, M.; Tibby, S.M.; Sykes, K.; Turner, C.; Murdoch, I.A. Diagnostic markers of infection: Comparison of procalcitonin with c reactive protein and leukocyte count. Arch. Dis. Child. 1999, 81, 417-421. [CrossRef] [PubMed] 39. Roers, A.; Hochkeppel, H.K.; Horisberger, M.A.; Hovanessian, A.; Haller, O. MxA gene expression after live virus vaccination: A sensitive marker for endogenous type I interferon. J. Infect. Dis. 1994, 169, 807-813.

[CrossRef] [PubMed] 40. Staeheli, P.; Pitossi, F.; Pa vlovic, J. Mx proteins: GTPases with antiviral activity. Trends Cell Biol. 1993, 3, 268-272. [CrossRef] 41. Staeheli, P. Interferon induced proteins and the antiviral state. Adv. Virus Res. 1990, 38, 147-200. [PubMed] 42. Chieux, V.; Hober, D.; Harvey, J.; Lion, G.; Lucidarme, D.; Forzy, G.; Duhamel, M.; Cousin, J.; Ducoulombier, H.; Wattré, P. The MxA protein levels in while blood lysates of patients with various viral infections. J. Virol. Methods 1998, 70, 183-191. [CrossRef] 43. Jain, S.; Self, W.H.; Wunderink, R.G.; Fakhran, S.; Balket, R.; Bramley, A.M.; Reed, C.; Grijalva, C.G.; Anderson, E.J.; Courtney, M.; et al. Community-acquired pneumonia requiring hospitalization among US adults. N. Engl. J. Med. 2015, 373, 415-427. [CrossRef] [PubMed] 44. Branche, A.R.; Walsh, E.E.; Vargas, R.; Hulbert, B.; Formica, M.A.; Baran, A.; Peterson, D.R.; Falsey, A.R.

Serum procalcitonin measurement and viral testing to guide antibiotic use for respiratory infections in hospitalized adults: A randomized controlled trial. J. Infect. Dis. 2015, 212, 1692-1700. [CrossRef] [PubMed] 45. Bosch, A.A.; Biesbroek, G.; Trzcinski, K.; Sanders, E.A.; Bogaert, D. Viral and bacterial interactions in the upper respiratory tract. PLoS Pathog. 2013, 9, e1003057. [CrossRef] [PubMed] 46. Kaplan, E.L.; Top, F.H., Jr.; Dudding, B.A.; Wannamaker, L.W. Diagnosis of streptococcal pharyngitis: Differentiation of active infection from the carrier state in the symptomatic child. J. Infect. Dis. 1971, 123, 490-501. [CrossRef] [PubMed] 47. Kaplan, E.L. The group A streptococcal upper respiratory tract carrier state: An enigma. J. Pediatr. 1980, 97, 337-345. [CrossRef] 48. Gerber, M.A.; Randolph, M.F.; Mayo, D.R. The group A streptococcal carrier state. A reexamination. Am. J.

Dis. Child. 1988, 142, 562-565. [CrossRef] [PubMed] 49. Komaroff, A.L.; Pass, T.M.; Aronson, M.D.; Ervin, C.T.; Cretin, S.; Winickoff, R.N.; Branch, W.T., Jr.

The prediction of streptococcal pharyngitis in adults. J. Gen. Intern. Med. 1986, 1, 1-7. [CrossRef] [PubMed] 50. Gerber, M.A.; Randolph, M.F.; Chanatry, J.; Wright, L.L.; DeMeo, K.K.; Anderson, L.R. Antigen detection test for streptococcal pharyngitis: Evaluation of sensitivity with respect totrue infection. J. Pediatr. 1986, 108, 654-658. [CrossRef] 51. Nussinovitch, M.; Finkelstein, Y.; Amir, J.; Varsano, I. Group A beta-hemolytic streptococcal pharyngitis in preschool children aged 3 months to 5 years. Clin. Pediatr. 1999, 38, 357-360. [CrossRef] [PubMed] 52. Ivaska, L.; Niemelä, J.; Lempainen, J.; Österback, R.; Warism, M.; Vuorinen, T.; Hytönen, J.; Rantakokko-Jalava, K.; Peltola, V. Aetiology of febrile pharyngitis in children: Potential of myxovirus resistance protein A (MxA) as a biomarker of viral infection. J. Infect. 2017, 74, 385-392. [CrossRef] [PubMed] 53. Mäkelä, M.J.; Puhakka, T.; Ruuskanen, O.; Leinonen, M.; Saikku, P.; Kimpimäki, M.; Blomqvist, S.; Hyypiä, T.; Arstila, P. Viruses and bacteria in the etiology of the common cold. J. Clin. Microbiol. 1998, 36, 539-542.

[PubMed] 54. Lingard, H.; Zehetmayer, S.; Maier, M. Bacterial superinfection in upper respiratory tract infections estimated by increases in CRP values: A diagnostic follow-up in primary care. Scand. J. Prim. Health Care 2008, 26, 211-215. [CrossRef] [PubMed] 55. Salonen, E.M.; Vaheri, A. C-Reactive protein in acute viral infections. J. Med. Virol. 1981, 8, 161-167.

[CrossRef] [PubMed] 56. Sarov, I.; Shainkin-Kestenbaum, R.; Zimlichman, R.; Winikoff, Y.; Chaimovitz, C.; Pras, M. Serum amyloid A levels in patients with infections due to cytomegalovirus, varicella-zoster virus and Herpes simplex virus.

J. Infect. Dis. 1982, 146, 443. [CrossRef] [PubMed] 57. Whicher, J.T.; Chambers, R.E.; Higginson, J.; Nashef, L.; Higgins, P.G. Acute phase response of serum amyloid A protein and C reactive protein to the common cold and influenza. J. Clin. Pathol. 1985, 38, 312-316.

[CrossRef] [PubMed] 58. Melbye, H.; Hvidsten, D.; Holm, A.; Nordbø, S.A.; Brox, J. The course of C-reactive protein response in untreated upper respiratory tract infection. Br. J. Gen. Pract. 2004, 54, 653-658. [PubMed] 59. Holm, A.; Pedersen, S.S.; Nexoe, J.; Obel, N.; Nielsen, L.P.; Koldkjaer, O.; Pedersen, C. Procalcitonin versus C-reactive protein for predicting pneumonia in adults with lower respiratory tract infection in primary care.

Br. J. Gen. Pract. 2007, 57, 555-560. [PubMed] 60. Kutz, A.; Briel, M.; Christ-Crain, M.; Stolz, D.; Bouadma, L.; Wolff, M.; Kristoffersen, K.B.; Wei, L.; Burkhardt, O.; Welte, T.; et al. Prognostic value of procalcitonin in respiratory tract infections across clinical settings. Crit. Care 2015, 19, 74. [CrossRef] [PubMed] 61. Kaplan, E.L.; Wannamaker, L.W. C-reactive protein in streptococcal pharyngitis. Pediatrics 1977, 60, 28-32.

23 references, page 1 of 2
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