publication . Article . 2021

Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli

Clément Madru; Ayten Dizkirici Tekpinar; Sandrine Rosario; Dariusz Czernecki; Sébastien Brûlé; Ludovic Sauguet; Marc Delarue;
Open Access English
  • Published: 29 Apr 2021
  • Publisher: HAL CCSD
  • Country: France
Abstract
<jats:p>To stop the COVID-19 pandemic due to the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which caused more than 2.5 million deaths to date, new antiviral molecules are urgently needed. The replication of SARS-CoV-2 requires the RNA-dependent RNA polymerase (RdRp), making RdRp an excellent target for antiviral agents. RdRp is a multi-subunit complex composed of 3 viral proteins named nsp7, nsp8 and nsp12 that ensure the ~30 kb RNA genome’s transcription and replication. The main strategies employed so far for the overproduction of RdRp consist of expressing and purifying the three subunits separately before assembling the complex <jats:itali...
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Subjects
Medical Subject Headings: viruses
free text keywords: [SDV]Life Sciences [q-bio], Research Article, Biology and life sciences, Organisms, Viruses, RNA viruses, Coronaviruses, SARS coronavirus, SARS CoV 2, Microbiology, Medical microbiology, Microbial pathogens, Viral pathogens, Medicine and health sciences, Pathology and laboratory medicine, Pathogens, Medical Conditions, Infectious Diseases, Viral Diseases, Covid 19, Research and Analysis Methods, Purification Techniques, Protein Purification, Recombinant Protein Purification, Molecular Biology, Molecular Biology Techniques, Artificial Gene Amplification and Extension, Primer Extension, Primer Extension Assay, Physical Sciences, Chemistry, Stoichiometry, Biochemistry, Proteins, Recombinant Proteins, Materials Science, Materials Physics, Sedimentation, Physics, Chemical Compounds, Organic Compounds, Imidazole, Organic Chemistry, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine
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COVID-19
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