publication . Article . 2014

Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity

Stefanie Gierer; Marcel A. Müller; Adeline Heurich; Daniel Ritz; Benjamin L. Springstein; Christina B. Karsten; Alexander Schendzielorz; Kerstin Gnirß; Christian Drosten; Stefan Pöhlmann;
Open Access English
  • Published: 23 Jul 2014 Journal: The Journal of Infectious Diseases, volume 211, issue 6, pages 889-897 (issn: 0022-1899, eissn: 1537-6613, Copyright policy)
  • Publisher: Oxford University Press
Abstract
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) infection is associated with a high case-fatality rate, and the potential pandemic spread of the virus is a public health concern. The spike protein of MERS-CoV (MERS-S) facilitates viral entry into host cells, which depends on activation of MERS-S by cellular proteases. Proteolytic activation of MERS-S during viral uptake into target cells has been demonstrated. However, it is unclear whether MERS-S is also cleaved during S protein synthesis in infected cells and whether cleavage is required for MERS-CoV infectivity. Here, we show that MERS-S is processed by proprotein convertases in MERS-S–transf...
Persistent Identifiers
Subjects
Medical Subject Headings: viruses
free text keywords: Major Articles and Brief Reports, Viruses, MERS-coronavirus, protease, TMPRSS2, trypsin, proprotein convertase, spike, activation, Immunology and Allergy, Infectious Diseases, Viral entry, Viral structural protein, Virology, Infectivity, Proprotein Convertases, Biology, Proteolytic enzymes, Coronavirus, medicine.disease_cause, medicine, Viral Receptor, Proprotein convertase
Funded by
EC| EMPERIE
Project
EMPERIE
European Management Platform for Emerging and Re-emerging Infectious disease Entities
  • Funder: European Commission (EC)
  • Project Code: 223498
  • Funding stream: FP7 | SP1 | HEALTH
Communities
COVID-19
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Update 16: summary and literature update as of 11 June 2014. 2014

Middle East respiratory syndrome coronavirus (MERS-CoV) - update. 2014

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