publication . Article . 2013

Investigation of Anti–Middle East Respiratory Syndrome Antibodies in Blood Donors and Slaughterhouse Workers in Jeddah and Makkah, Saudi Arabia, Fall 2012

Asad S. Aburizaiza; Frank M. Mattes; Esam I. Azhar; Ahmed M. Hassan; Ziad A. Memish; Doreen Muth; Benjamin Meyer; Erik Lattwein; Marcel A. Müller; Christian Drosten;
Open Access English
  • Published: 11 Nov 2013 Journal: The Journal of Infectious Diseases, volume 209, issue 2, pages 243-246 (issn: 0022-1899, eissn: 1537-6613, Copyright policy)
  • Publisher: Oxford University Press
Abstract
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel, potentially zoonotic human coronavirus (HCoV). We investigated MERS-CoV antibodies using a staged approach involving an immunofluorescence assay (IFA), a differential recombinant IFA, and a plaque-reduction serum neutralization assay. In 130 blood donors sampled during 2012 in Jeddah and 226 slaughterhouse workers sampled in October 2012 in Jeddah and Makkah, Saudi Arabia, 8 reactive sera were seen in IFA but were resolved to be specific for established HCoVs by discriminative testing. There is no evidence that MERS-CoV circulated widely in the study region in fall 2012, matching an app...
Persistent Identifiers
Subjects
Medical Subject Headings: virus diseasesparasitic diseasesviruses
free text keywords: Major Articles and Brief Reports, Viruses, Editor's Choice, MERS-Coronavirus, serology, population immunity, Immunology and Allergy, Infectious Diseases, Neutralization test, Antibody, biology.protein, biology, Middle East respiratory syndrome coronavirus, medicine.disease_cause, medicine, Serology, Seroepidemiologic Studies, Virology, Middle East respiratory syndrome, medicine.disease, Hajj, business.industry, business, Coronavirus

Memish, ZA, Zumla, AI, Al-Hakeem, RF, Al-Rabeeah, AA, Stephens, GM. Family cluster of middle east respiratory syndrome coronavirus infections. N Engl J Med. 2013; 368: 2487-94 [OpenAIRE] [PubMed]

Cauchemez, S, Epperson, S, Biggerstaff, M, Swerdlow, D, Finelli, L, Ferguson, NM. Using routine surveillance data to estimate the epidemic potential of emerging zoonoses: application to the emergence of US swine origin influenza A H3N2v virus. PLoS Med. 2013; 10: e1001399 [OpenAIRE] [PubMed]

Drosten, C, Seilmaier, M, Corman, VM. Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect Dis. 2013; 13: 745-51 [OpenAIRE] [PubMed]

Reusken, CB, Haagmans, BL, Muller, MA. Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 2013

Chan, KH, Chan, JF, Tse, H. Cross-reactive antibodies in convalescent SARS patients’ sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests. J Infect. 2013; 67: 130-40 [OpenAIRE] [PubMed]

Buchholz, U, Muller, MA, Nitsche, A. Contact investigation of a case of human novel coronavirus infection treated in a German hospital, October-November 2012. Euro Surveill. 2013; 18

de Groot, RJ, Baker, SC, Baric, RS. Middle east respiratory syndrome coronavirus (MERS-CoV); announcement of the coronavirus study group. J Virol. 2013; 87: 7790-2 [OpenAIRE] [PubMed]

Corman, V, Muller, M, Costabel, U. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill. 2012; 17

Zaki, AM, van Boheemen, S, Bestebroer, TM, Osterhaus, AD, Fouchier, RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012; 367: 1814-20 [OpenAIRE] [PubMed]

Herzog, P, Drosten, C, Muller, MA. Plaque assay for human coronavirus NL63 using human colon carcinoma cells. Virol J. 2008; 5: 138 [OpenAIRE] [PubMed]

Al-Tawfiq, JA, Memish, ZA. The Hajj: updated health hazards and current recommendations for 2012. Euro Surveill. 2012; 17: 20295 [PubMed]

Benkouiten, S, Charrel, R, Belhouchat, K. Circulation of respiratory viruses among pilgrims during the 2012 Hajj pilgrimage. Clin Infect Dis. 2013; 57: 992-1000 [OpenAIRE] [PubMed]

Gautret, P, Charrel, R, Belhouchat, K. Lack of nasal carriage of novel corona virus (HCoV-EMC) in French Hajj pilgrims returning from the Hajj 2012, despite a high rate of respiratory symptoms. Clin Microbiol Infect. 2013; 19: E315-7 [OpenAIRE] [PubMed]

Any information missing or wrong?Report an Issue