publication . Article . 2020

Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR

Eelke Brandsma; Han J.M.P. Verhagen; Thijs J.W. van de Laar; Eric C. J. Claas; Marion Cornelissen; Emile van den Akker;
Open Access English
  • Published: 10 Oct 2020 Journal: Journal of infectious diseases, volume 223, issue 2, pages 206-213 (issn: 0022-1899, Copyright policy)
Abstract
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Recent advances in CRISPR-based diagnostics suggest that DETECTR, a combination of reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) and subsequent Cas12 bystander nuclease activation by amplicon-targeting ribonucleoprotein complexes, could be a faster and cheaper alternative to quantitative reverse-transcription polymerase chain reaction (qRT-PCR) without sacrificing sensitivity and/or specificity.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>In this study, we compare DETECTR with qRT-PCR to diagnose coronavirus disease 2019 ...
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Subjects
free text keywords: COVID-19, DETECTR, SARS-CoV-2, qRT-PCR, Immunology and Allergy, Infectious Diseases, Reverse transcriptase, Nuclease, biology.protein, biology, Medicine, business.industry, business, Polymerase chain reaction, law.invention, law, Real-time polymerase chain reaction, CRISPR, RNA-Directed DNA Polymerase, Amplicon, Virology, Point-of-care testing
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