publication . Article . Other literature type . 2018

Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion

Mauthe, Mario; Orhon, Idil; Rocchi, Cecilia; Zhou, Xingdong; Luhr, Morten; Hijlkema, Kerst-Jan; Coppes, Robert P.; Engedal, Nikolai; Mari, Muriel; Reggiori, Fulvio;
Open Access English
  • Published: 20 Jul 2018
  • Country: Netherlands
Abstract
Macroautophagy/autophagy is a conserved transport pathway where targeted structures are sequestered by phagophores, which mature into autophagosomes, and then delivered into lysosomes for degradation. Autophagy is involved in the pathophysiology of numerous diseases and its modulation is beneficial for the outcome of numerous specific diseases. Several lysosomal inhibitors such as bafilomycin A(1) (BafA(1)), protease inhibitors and chloroquine (CQ), have been used interchangeably to block autophagy in in vitro experiments assuming that they all primarily block lysosomal degradation. Among them, only CQ and its derivate hydroxychloroquine (HCQ) are FDA-approved d...
Subjects
free text keywords: Autophagy, bafilomycin A(1), degradative compartments, fusion, Golgi, lysosomal degradation, lysosomal inhibitors, MANNOSE 6-PHOSPHATE RECEPTOR, INTEGRAL MEMBRANE-PROTEIN, ISOLATED RAT HEPATOCYTES, GROWTH-FACTOR RECEPTOR, NONSELECTIVE AUTOPHAGY, PERITONEAL-MACROPHAGES, INTRALYSOSOMAL PH, LC3 LIPIDATION, SELF-DIGESTION, CANCER-CELLS, Cell Biology, Molecular Biology, Resource, Autophagy, Cancer cell, Mannose 6-phosphate receptor, Biology, Chloroquine, medicine.drug, medicine, Golgi apparatus, symbols.namesake, symbols, Integral membrane protein, Transport Pathway, Growth factor receptor, Cell biology
Communities
  • COVID-19
  • Social Science and Humanities
Funded by
EC| PRONKJEWAIL
Project
PRONKJEWAIL
Protecting patients with enhanced susceptibility to infections
  • Funder: European Commission (EC)
  • Project Code: 713660
  • Funding stream: H2020 | MSCA-COFUND-DP
,
SNSF| ER-phagy mechanisms to maintain and restore endoplasmic reticulum homeostasis
Project
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: CRSII3_154421
  • Funding stream: Programmes | Sinergia
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Other literature type . 2018
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