publication . Article . 2021

Long-term evolution of the epithelial cell secretome in preclinical 3D models of the human bronchial epithelium

Daniel Sanchez-Guzman; Sonja Boland; Oliver Brookes; Claire Mc Cord; René Lai Kuen; Valentina Sirri; Armelle Baeza Squiban; Stéphanie Devineau;
Open Access English
  • Published: 23 Mar 2021 Journal: Scientific Reports, volume 11 (eissn: 2045-2322, Copyright policy)
  • Publisher: Nature Publishing Group UK
  • Country: France
Abstract
AbstractThe human bronchial epithelium is the first line of defense against atmospheric particles, pollutants, and respiratory pathogens such as the novel SARS-CoV-2. The epithelial cells form a tight barrier and secrete proteins that are major components of the mucosal immune response. Functional in vitro models of the human lung are essential for screening the epithelial response and assessing the toxicity and barrier crossing of drugs, inhaled particles, and pollutants. However, there is a lack of models to investigate the effect of chronic exposure without resorting to animal testing. Here, we developed a 3D model of the human bronchial epithelium using Calu-3 cell line and demonstrated its viability and functionality for 21 days without subculturing. We investigated the effect of reduced Fetal Bovine Serum supplementation in the basal medium and defined the minimal supplementation needed to maintain a functional epithelium, so that the amount of exogenous serum proteins could be reduced during drug testing. The long-term evolution of the epithelial cell secretome was fully characterized by quantitative mass spectrometry in two preclinical models using Calu-3 or primary NHBE cells. 408 common secreted proteins were identified while significant differences in protein abundance were observed with time, suggesting that 7–10 days are necessary to establish a mature secretome in the Calu-3 model. The associated Reactome pathways highlight the role of the secreted proteins in the immune response of the bronchial epithelium. We suggest this preclinical 3D model can be used to evaluate the long-term toxicity of drugs or particles on the human bronchial epithelium, and subsequently to investigate their effect on the epithelial cell secretions.
Persistent Identifiers

doi: 10.1038/s41598-021-86037-0

pmc: PMC7988136

pmid: 33758289

Fields of Science and Technology classification (FOS)
03 medical and health sciences, 0301 basic medicine, 0302 clinical medicine, 030220 oncology & carcinogenesis, 030304 developmental biology
Sustainable Development Goals (SDG)
13. Climate action
Subjects
free text keywords: Article, Cell biology, Proteomics, Respiratory system models, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV]Life Sciences [q-bio], Multidisciplinary, Medicine, R, Science, Q, Epithelium, medicine.anatomical_structure, medicine, Blood proteins, Proteomics, Fetal bovine serum, Cell biology, Secretory protein, Biology, Immune system, Cell culture, Secretion
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Communities
  • COVID-19
Funded by
EC| BIORIMA
Project
BIORIMA
BIOmaterial RIsk MAnagement
  • Funder: European Commission (EC)
  • Project Code: 760928
  • Funding stream: H2020 | RIA
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