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description Publicationkeyboard_double_arrow_right Article 2016Embargo end date: 01 Apr 2016 France, Switzerland, Switzerland EnglishPublisher:ETH Zurich Paola De Benedictis; Andrea Minola; Elena Rota Nodari; Roberta Aiello; Barbara Zecchin; Angela Salomoni; Mathilde Foglierini; Gloria Agatic; Fabrizia Vanzetta; Rachel Lavenir; Anthony Lepelletier; Emma M. Bentley; Robin A. Weiss; Giovanni Cattoli; Ilaria Capua; Federica Sallusto; Edward Wright; Antonio Lanzavecchia; Hervé Bourhy; Davide Corti;Currently available rabies post‐exposure prophylaxis (PEP) for use in humans includes equine or human rabies immunoglobulins (RIG). The replacement of RIG with an equally or more potent and safer product is strongly encouraged due to the high costs and limited availability of existing RIG. In this study, we identified two broadly neutralizing human monoclonal antibodies that represent a valid and affordable alternative to RIG in rabies PEP. Memory B cells from four selected vaccinated donors were immortalized and monoclonal antibodies were tested for neutralizing activity and epitope specificity. Two antibodies, identified as RVC20 and RVC58 (binding to antigenic site I and III, respectively), were selected for their potency and broad‐spectrum reactivity. In vitro, RVC20 and RVC58 were able to neutralize all 35 rabies virus (RABV) and 25 non‐RABV lyssaviruses. They showed higher potency and breath compared to antibodies under clinical development (namely CR57, CR4098, and RAB1) and commercially available human RIG. In vivo, the RVC20–RVC58 cocktail protected Syrian hamsters from a lethal RABV challenge and did not affect the endogenous hamster post‐vaccination antibody response. EMBO Molecular Medicine, 8 (4) ISSN:1757-4684 ISSN:1757-4676
Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4818751Data sources: PubMed CentralHAL-Pasteur; Mémoires en Sciences de l'Information et de la CommunicationArticle . 2016License: CC BYadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3929/ethz-b-000114473&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 71 citations 71 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!visibility 4visibility views 4 download downloads 42 Powered bymore_vert Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4818751Data sources: PubMed CentralHAL-Pasteur; Mémoires en Sciences de l'Information et de la CommunicationArticle . 2016License: CC BYadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3929/ethz-b-000114473&type=result"></script>'); --> </script>
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description Publicationkeyboard_double_arrow_right Article 2016Embargo end date: 01 Apr 2016 France, Switzerland, Switzerland EnglishPublisher:ETH Zurich Paola De Benedictis; Andrea Minola; Elena Rota Nodari; Roberta Aiello; Barbara Zecchin; Angela Salomoni; Mathilde Foglierini; Gloria Agatic; Fabrizia Vanzetta; Rachel Lavenir; Anthony Lepelletier; Emma M. Bentley; Robin A. Weiss; Giovanni Cattoli; Ilaria Capua; Federica Sallusto; Edward Wright; Antonio Lanzavecchia; Hervé Bourhy; Davide Corti;Currently available rabies post‐exposure prophylaxis (PEP) for use in humans includes equine or human rabies immunoglobulins (RIG). The replacement of RIG with an equally or more potent and safer product is strongly encouraged due to the high costs and limited availability of existing RIG. In this study, we identified two broadly neutralizing human monoclonal antibodies that represent a valid and affordable alternative to RIG in rabies PEP. Memory B cells from four selected vaccinated donors were immortalized and monoclonal antibodies were tested for neutralizing activity and epitope specificity. Two antibodies, identified as RVC20 and RVC58 (binding to antigenic site I and III, respectively), were selected for their potency and broad‐spectrum reactivity. In vitro, RVC20 and RVC58 were able to neutralize all 35 rabies virus (RABV) and 25 non‐RABV lyssaviruses. They showed higher potency and breath compared to antibodies under clinical development (namely CR57, CR4098, and RAB1) and commercially available human RIG. In vivo, the RVC20–RVC58 cocktail protected Syrian hamsters from a lethal RABV challenge and did not affect the endogenous hamster post‐vaccination antibody response. EMBO Molecular Medicine, 8 (4) ISSN:1757-4684 ISSN:1757-4676
Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4818751Data sources: PubMed CentralHAL-Pasteur; Mémoires en Sciences de l'Information et de la CommunicationArticle . 2016License: CC BYadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3929/ethz-b-000114473&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 71 citations 71 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!visibility 4visibility views 4 download downloads 42 Powered bymore_vert Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4818751Data sources: PubMed CentralHAL-Pasteur; Mémoires en Sciences de l'Information et de la CommunicationArticle . 2016License: CC BYadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3929/ethz-b-000114473&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu