SummaryBackgroundCOVID-19 booster vaccine uptake rates are behind the rate of primary vaccination in many countries. Governments and non-governmental institutions rely on a range of interventions aiming to increase booster uptake. Yet, little is known how experts and the general public evaluate these interventions.MethodsWe applied a novel crowdsourcing approach to provide rapid insights on the most promising interventions to promote uptake of COVID-19 booster vaccines. In the first phase (December 2021), international experts (n = 78 from 17 countries) proposed 46 unique interventions. To reduce noise and potential bias, in the second phase (January 2022), experts (n = 307 from 34 countries) and representative general population samples from the UK (n = 299) and the US (n = 300) rated the proposed interventions on several evaluation criteria, including effectiveness and acceptability, on a 5-point Likert-type scale.FindingsSanctions were evaluated as potentially most effective but least accepted. Evaluations by expert and general population samples were considerably aligned. Interventions that received the most positive evaluations regarding both effectiveness and acceptability across evaluation groups were: a day off work after getting vaccinated, financial incentives, tax benefits, promotional campaigns, and mobile vaccination teams.InterpretationThe results provide useful insights to help governmental and non-governmental institutions in their decisions about which interventions to implement. Additionally, the applied crowdsourcing method may be used in future studies to retrieve rapid insights on the comparative evaluation of (health) policies.FundingThis study received funding from the Austrian Science Fund (SFB F63) and the University of Vienna.
Understanding predictors of adherence to governmental measures to prevent the spread of the COVID-19 is fundamental to guide health communication. This study examined whether political stringency and infection rates during the first wave of the pandemic were associated with higher education students' adherence to COVID-19 government measures in the Nordic countries (Denmark, Finland, Norway, Iceland, and Sweden) and the United Kingdom. Both individual- and country-level data were used in present study. An international cross-sectional subsample (n = 10,345) of higher-education students was conducted in May–June 2020 to collect individual-level information on socio-demographics, study information, living arrangements, health behaviors, stress, and COVID-19-related concerns, including adherence to government measures. Country-level data on political stringency from the Oxford COVID-19 Government Response Tracker and national infection rates were added to individual-level data. Multiple linear regression analyses stratified by country were conducted. Around 66% of students reported adhering to government measures, with the highest adherence in the UK (73%) followed by Iceland (72%), Denmark (69%), Norway (67%), Finland (64%) and Sweden (49%). Main predictors for higher adherence were older age, being female and being worried about getting infected with COVID-19 (individual-level), an increase in number of days since lockdown, political stringency, and information about COVID-19 mortality rates (country-level). However, incidence rate was an inconsistent predictor, which may be explained by imperfect data quality during the onset of the pandemic. We conclude that shorter lockdown periods and political stringency are associated with adherence to government measures among higher education students at the outset of the COVID-19 pandemic.
Countries: Australia, United Kingdom, Denmark, Australia, Denmark
Following the resurgence of the COVID-19 epidemic in the UK in late 2020 and the emergence of the alpha (also known as B117) variant of the SARS-CoV-2 virus, a third national lockdown was imposed from January 4, 2021. Following the decline of COVID-19 cases over the remainder of January 2021, the question of when and how to reopen schools became an increasingly pressing one in early 2021. This study models the impact of a partial national lockdown with social distancing measures enacted in communities and workplaces under different strategies of reopening schools from March 8, 2021 and compares it to the impact of continual full national lockdown remaining until April 19, 2021. We used our previously published agent-based model, Covasim, to model the emergence of the alpha variant over September 1, 2020 to January 31, 2021 in presence of Test, Trace and Isolate (TTI) strategies. We extended the model to incorporate the impacts of the roll-out of a two-dose vaccine against COVID-19, with 200,000 daily vaccine doses prioritised by age starting with people 75 years or older, assuming vaccination offers a 95% reduction in disease acquisition risk and a 30% reduction in transmission risk. We used the model, calibrated until January 25, 2021, to simulate the impact of a full national lockdown (FNL) with schools closed until April 19, 2021 versus four different partial national lockdown (PNL) scenarios with different elements of schooling open: 1) staggered PNL with primary schools and exam-entry years (years 11 and 13) returning on March 8, 2021 and the rest of the schools years on March 15, 2020; 2) full-return PNL with both primary and secondary schools returning on March 8, 2021; 3) primary-only PNL with primary schools and exam critical years (years 11 and 13) going back only on March 8, 2021 with the rest of the secondary schools back on April 19, 2021 and 4) part-rota PNL with both primary and secondary schools returning on March 8, 2021 with primary schools remaining open continuously but secondary schools on a two-weekly rota-system with years alternating between a fortnight of face-to-face and remote learning until April 19, 2021. Across all scenarios, we projected the number of new daily cases, cumulative deaths and effective reproduction number R until April 30, 2021. Our calibration across different scenarios is consistent with alpha variant being around 60% more transmissible than the wild type. We find that strict social distancing measures, i.e. national lockdowns, were essential in containing the spread of the virus and controlling hospitalisations and deaths during January and February 2021. We estimated that a national lockdown over January and February 2021 would reduce the number of cases by early March to levels similar to those seen in October 2020, with R also falling and remaining below 1 over this period. We estimated that infections would start to increase when schools reopened, but found that if other parts of society remain closed, this resurgence would not be sufficient to bring R above 1. Reopening primary schools and exam critical years only or having primary schools open continuously with secondary schools on rotas was estimated to lead to lower increases in cases and R than if all schools opened. Without an increase in vaccination above the levels seen in January and February, we estimate that R could have increased above 1 following the reopening of society, simulated here from April 19, 2021. Our findings suggest that stringent measures were integral in mitigating the increase in cases and bringing R below 1 over January and February 2021. We found that it was plausible that a PNL with schools partially open from March 8, 2021 and the rest of the society remaining closed until April 19, 2021 would keep R below 1, with some increase evident in infections compared to continual FNL until April 19, 2021. Reopening society in mid-April, without an increase in vaccination levels, could push R above 1 and induce a surge in infections, but the effect of vaccination may be able to control this in future depending on the transmission blocking properties of the vaccines.
Maximilian G. R. Vollstädt; Mauro Galetti; Christopher N. Kaiser‐Bunbury; Benno I. Simmons; Fernando Gonçalves; Alcides L. Morales‐Pérez; Luis Navarro; Fabio L. Tarazona‐Tubens; Spencer Schubert; Tomas Carlo; +6 more
Maximilian G. R. Vollstädt; Mauro Galetti; Christopher N. Kaiser‐Bunbury; Benno I. Simmons; Fernando Gonçalves; Alcides L. Morales‐Pérez; Luis Navarro; Fabio L. Tarazona‐Tubens; Spencer Schubert; Tomas Carlo; Jackeline Salazar; Michel Faife‐Cabrera; Allan Strong; Hannah Madden; Adam Mitchell; Bo Dalsgaard;
AimMutualistic interactions between plants and animals are fundamental for the maintenance of natural communities and the ecosystem services they provide. However, particularly in human-dominated island ecosystems, introduced species may alter mutualistic interactions. Based on an extensive dataset of plant–frugivore interactions, we mapped and analysed a meta-network across the Caribbean archipelago. Specifically, we searched for subcommunity structure (modularity) and identified the types of species facilitating the integration of introduced species in the Caribbean meta-network.LocationCaribbean archipelago (Lucayan archipelago, Greater Antilles, Lesser Antilles).MethodsWe reviewed published scientific literature, unpublished theses and other nonpeer-reviewed sources to compile an extensive dataset of plant–frugivore interactions. We visualized spatial patterns and conducted a modularity analysis of the cross-island meta-network. We also examined which species were most likely to interact with introduced species: (1) endemic, nonendemic native or introduced species, and (2) generalized or specialized species.ResultsWe reported 3060 records of interactions between 486 plant and 178 frugivore species. The Caribbean meta-network was organized in 13 modules, driven by a combination of functional or taxonomic (modules dominated by certain groups of frugivores) and biogeographical (island-specific modules) mechanisms. Few introduced species or interaction pairs were shared across islands, suggesting little homogenization of the plant–frugivore meta-network at the regional scale. However, we found evidence of “invader complexes,” as introduced frugivores were more likely to interact with introduced plants than expected at random. Moreover, we found generalist species more likely to interact with introduced species than were specialized species.Main conclusionsThese results demonstrate that generalist species and “invader complexes” may facilitate the incorporation of introduced species into plant–frugivore communities. Despite the influx of introduced species, the meta-network was structured into modules related to biogeographical and functional or taxonomic affinities. These findings reveal how introduced species become an integral part of mutualistic systems on tropical islands.1 INTRODUCTIONMutualistic interactions between plants and animals, such as pollinators and frugivores, are critically important for maintaining the functionality of natural communities (Jordano, 1987;Ollerton et al., 2011; Rech et al., 2016). While most flowering plants are dependent on animals for pollination and seed-set (Ollerton et al., 2011; Rech et al., 2016), animal frugivores may ingest or otherwise manipulate and consequently disperse millions of seeds annually (Bueno et al., 2013). Frugivory is thereby crucial for the maintenance of plant diversity (Harms et al., 2000), as it allows plants to populate new sites, maintains gene flow between distinct populations and decreases density-dependent mortality in proximity of the parent individuals (Rogers et al., 2021). In some tropical systems, approximately 90% of all woody plants depend on frugivores for seed dispersal (Almeida-Neto et al., 2008; Howe & Smallwood, 1982). In addition to providing direct dispersal to specific, favourable sites for the plant (Wenny & Levey, 1998), frugivores can enhance the probability of successful germination, for example through the passage of seeds in the intestinal system (e.g. Traveset et al., 2001). The most important frugivore groups are birds, mammals and reptiles with birds and reptiles being particularly important in tropical island ecosystems (Kaiser-Bunbury et al., 2010; Valido & Olesen, 2007).Globally, co-evolved plant–frugivore communities are suffering from an array of drivers associated with global change, such as the introduction of species into new environments, where they become integrated into local communities through species interactions (Gallardo et al., 2016; Vilà et al., 2011). Species communities are thus being altered, which in turn may have consequences for biotic interactions and ecosystem functions, such as seed dispersal (Aslan et al., 2013; Lugo et al., 2012; Traveset & Richardson, 2006; Vizentin-Bugoni et al., 2021). Island ecosystems are particularly vulnerable to the disruption of native plant–frugivore interactions as island mutualists have evolved in isolation, and frequently developed specific traits, such as altered dispersal, or loss of defence traits in plants (Burns, 2019). Furthermore, as islands harbour many endemic species found nowhere else on Earth (Kier et al., 2009; Paulay, 1994), and have experienced disproportionally high extinction rates and numerous extant island species are threatened with extinctions (Blackburn et al., 2004; Fernández-Palacios et al., 2021; Groombridge, 1992), it is especially important to understand how introduced species integrate into island communities (Wood et al., 2017).Introduced species may integrate into existing communities and establish themselves in different ways. For instance, the concept of “invader complexes” suggests that introduced species facilitate the establishment of other introduced species, resulting in groups of introduced species interacting strongly with each other and less with the remaining community (D'Antonio & Dudley, 1993). Alternatively, endemic species that have become superabundant and highly generalized species due to ecological release and density compensation may readily include new arrivals into their interactions and thereby facilitate the establishment of introduced species on islands (Olesen et al., 2002). Furthermore, a growing number of studies show that species with many mutualistic partners (i.e. generalized species irrespectively of being nonendemic native or endemic) are more likely to incorporate new partners into their networks (Bascompte & Stouffer, 2009; Maruyama et al., 2016). In network theory, this is called “preferential attachment” (Newman, 2001), and thus, most generalized species would be expected to interact with introduced species.In addition to understanding which species are responsible for incorporating introduced species into native communities, we have little quantitative understanding of how introduced species affect the structure of native interaction networks and how this varies biogeographically (Fricke & Svenning, 2020). As for other mutualistic networks, plants and frugivores form complex interaction networks with reccurring structural properties (Bascompte & Jordano, 2007). One such property of interaction networks is modularity, which describes how interacting species aggregate into modules consisting of species that interact strongly within the respective module but much less with species of other modules (Thébault, 2013). The modular structure of mutualistic networks may reflect “co-evolutionary units” (Olesen et al., 2007) determined by an array of factors, such as phenological overlap, morphological traits, taxonomic relatedness and biogeography (Araujo et al., 2018; Dalsgaard et al., 2013; Donatti et al., 2011; Martín González et al., 2018; Maruyama et al., 2014; Schleuning et al., 2014). However, it is poorly understood whether introduced species influence the modular structure of mutualistic systems.Here, we present an extensive dataset on plant–frugivore interactions compiled from published and unpublished resources across the islands of the Caribbean archipelago: Lucayan archipelago, Greater Antilles and Lesser Antilles. We use the data to (i) explore the distribution of frugivory records across the Caribbean islands; (ii) assess island connectivity through shared species and interactions; (iii) evaluate the modular structure of the regional plant–frugivore meta-network and (iv) determine whether generalized vs. specialized species and introduced vs. endemic species are more likely to integrate introduced plants and frugivores into native plant–frugivore communities in island systems.2 METHODS2.1 Data collection and study regionAll our data were collected on the Caribbean islands, that is the Lucayan archipelago (The Bahamas and Turks and Caicos), the Greater Antilles (Cuba, Cayman Islands, Jamaica, Hispaniola and Puerto Rico) and the Lesser Antilles (a series of islands from the US and British Virgin Islands in the north to Grenada in the south). We did not include plant–frugivore interactions from islands such as Trinidad and Tobago, Curaçao, and Bonaire just north of South America, as these are continental islands with biotas with strong affinities to the South American mainland (Carstensen et al., 2012; Ricklefs & Bermingham, 2008). The low-lying sedimentary islands of the Lucayan Archipelago are part of the North American platform (Iturralde-Vinent & MacPhee, 1999; Trejo-Torres & Ackerman, 2001), and some of the islands have been interconnected in the Pleistocene (Murphy et al., 2004; Trejo-Torres & Ackerman, 2001). The mostly large and mountainous islands of the Greater Antilles are old with different geological origins (Graham, 2003; Iturralde-Vinent & MacPhee, 1999). The Greater Antilles emerged as fragments in the Eocene about 49 Ma; the geological history of the region has been highly dynamic with some parts connected in the past (Buskirk, 1985; Graham, 2003; Iturralde-Vinent & MacPhee, 1999; Ricklefs & Bermingham, 2008). The current biota of the Greater Antilles was only in small parts formed by vicariance, with dispersal facilitated by the Aves Ridge about 32–35 Ma (Iturralde-Vinent & MacPhee, 1999) or a more likely overwater dispersal at least for the avifauna (Buskirk, 1985; Graham, 2003; Ricklefs & Bermingham, 2008). The Lesser Antilles form a volcanic arc where the North and South American plates subduct under the Caribbean plate and likely originated at least 20 Ma (Ricklefs & Bermingham, 2008). To the east of the volcanic arc are some younger and low-lying islands such as Antigua and Barbuda, which consist of uplifted marine sediments (Ricklefs & Bermingham, 2008; Ricklefs & Lovette, 1999). Some islands were interconnected during the last glacial maximum, but most Lesser Antilles islands have never been interconnected (Ricklefs & Bermingham, 2004, 2008). The isolation of the Caribbean islands from the mainland differs greatly (Carstensen et al., 2012). Bimini in the Bahamas, for instance, is only approx. 87 km from the North American continent and Grenada in the Lesser Antilles is only 137 km from the continental landmass of South America. By contrast, islands such as Grand Turk (993 km) and South Caicos (999 km) are much more isolated from the mainland. On average, the isolation from any continental landmass in the Caribbean is over 500 km (Mean: 593 km ± 248 km SD; see details in Supporting Information Table S1). The distances between single islands are much smaller, for example the distance between Martinique and Dominica and Martinique and Saint Lucia is approx. 40 km. An island size threshold of 10,000 km2 has previously been suggested to be important for islands to be considered sources for colonization (Weigelt & Kreft, 2013), and on average, the islands of the Caribbean are approx. 304 km (±174 km SD) from the nearest island that exceeds 10,000 km2 (Table S1). Given the geological history and isolation of the Caribbean, the biota is characterized by being depauperate with high levels of endemism.To collect data on interactions between plants and frugivores in the Caribbean, we screened the Web of Science (WoS) and Google Scholar search engines. We used the combination of the following search terms: (“frugivory” OR “seed dispersal” OR “seed removal” OR “mutualism”) AND (“Caribbean” OR “Lesser Antilles” OR “Greater Antilles” OR “West Indies” OR “Bahamas” OR “Turks and Caicos” OR “Cayman Islands” OR “Jamaica” OR “Cuba” OR “Hispaniola” OR “Haiti” OR “Dominican Republic” OR “República Dominicana” OR “Puerto Rico” OR “Mona” OR “Virgin Islands” OR “Saint Martin” OR “Anguilla” OR “St. Kitts and Nevis” OR “Antigua” OR “Barbuda” OR “Montserrat” OR “Guadeloupe” OR “Dominica” OR “Martinique” OR “St. Lucia” OR “St. Vincent” OR “Grenadines” OR “Barbados” OR “Grenada”). To also include the grey literature, we contacted local ornithologists and ecologists working in the Caribbean region. This approach allowed us to obtain non-English publications, such as theses and dissertations not available online. We screened each of the studies manually, discarding studies where no appropriate data were presented (e.g. mutualistic interactions in marine environments). Interactions were only included when the respective authors presented original evidence for interaction events, that is evidence of fruits and/or seeds being ingested by frugivores. Thus, we discarded records where interactions between species were speculative (e.g. observation of frugivores on fruiting plant species without any evidence of fruit ingestion).We standardized the species names of plants and frugivores using the R-package taxize (Chamberlain & Szocs, 2013; Global Names Resolver, 2021) and data from the Integrated Taxonomic Information System (ITIS, 2021). We also retrieved information about species taxonomies (i.e. class, order and family) from ITIS. Finally, we compiled information about the native status of species and classified them into nonendemic native (species native to the Caribbean, but also naturally occurring elsewhere), endemic (only occurring within the Caribbean) and introduced (not naturally occurring within Caribbean) species (see details in Supporting Information Text S1). Of the original records, 95 plant (approx. 16% of all reported plants) and one frugivore record were not identified to species level (e.g. only genus name reported) and were thus excluded from data analyses. The final data used in statistical analyses consisted of interactions between 486 plant and 178 frugivore species.2.2 Data analysis2.2.1 Cross-island patterns of shared species and interactionsWe summarized patterns of shared species and interaction pairs across the Caribbean by calculating the proportion of shared species and interaction pairs across all islands. We calculated this proportion as the number of species/interaction pairs found on any two islands, divided by the total number of species/interaction pairs found on the given islands (Fricke & Svenning, 2020). We summarized these patterns separately for all reported records, for endemic, nonendemic native and for introduced plant and frugivore species and interaction pairs, respectively.2.2.2 Modularity of the Caribbean plant–frugivore meta-networkTo detect a modular structure of the meta-network, that is the network of plant–frugivore interactions across all islands, we employed Beckett's DIRT-LPA algorithm in the computeModules function of the R-package “bipartite” (Dormann et al., 2008, 2009). We ran 10 independent runs of the algorithm on the binary meta-network containing interactions between all identified species and identified the run with the single best division into modules, that is the highest degree of modularity Q. For the run with the highest Q value, we recorded the Q value, the number of modules as well as the respective plant and frugivore species in each module (Schleuning et al., 2014) and the islands on which they were recorded. To test whether the identified modular structure of the meta-network differed from random, we compared our results to 100 null models. To this end, we used an algorithm proposed by Patefield (1981) to randomize the interactions between species, using fixed marginal totals to produce networks with randomly associated species without constraining the degree of specialization (Blüthgen et al., 2008; Schleuning et al., 2014). For each of the null models, we applied the same approach as with the original matrix, that is we identified the single best configuration from 10 independent runs (Schleuning et al., 2014). We then tested whether modularity of the original matrix was significantly different from the best 100 null models by looking at the proportion of null modularity values that were greater than the empirical one, that is if .05; Figure S2c) and the frugivore perspective (slope = 0.003, p > .05; Figure S2d).4 DISCUSSIONHere, we present a comprehensive review of published plant–frugivore interactions across the Caribbean archipelago, including the Lucayan archipelago, the Greater and Lesser Antilles. All islands shared species and unique interaction pairs with neighbouring islands and archipelagos, thereby forming a cohesive meta-network. We show that the meta-network of plant–frugivore interactions across the Caribbean was structured into modules, with at least some modules determined by a combination of functional or taxonomic (i.e. certain groups of frugivores) and biogeographical (i.e. island-specific modules) mechanisms. While relatively few species in the dataset were introduced to the Caribbean (16% plant and 8% frugivore species), we found support for the “invader complexes” theory, whereby introduced species facilitate the establishment of other introduced species (D'Antonio & Dudley, 1993; Olesen et al., 2002). Moreover, we found that generalized species were more likely to incorporate introduced species into their interactions, giving support for the “preferential attachment” theory (Newman, 2001). Below, we first discuss the available data on frugivory in the Caribbean, whereafter we discuss how species and interactions are shared across islands. We end by discussing the drivers of modularity and the integration of introduced species into plant–frugivore communities across the Caribbean.4.1 Data on frugivores and their plants in the Caribbean archipelagoAcross all islands, the vast majority of reported frugivores were birds (79%), followed by reptiles (13%) and mammals (8%), of which in turn the majority were bats (71%). These data thus reflect patterns that are typical for oceanic islands, as there is generally a lack of nonvolant, large-bodied, frugivorous mammals which may be ecologically replaced by birds and reptiles (Kaiser-Bunbury et al., 2010). The low number of mammal species in the dataset could also reflect past mammal extinctions particularly on the islands of the Greater Antilles (Turvey et al., 2021), potentially leaving some plants without their main seed dispersers.A large proportion of the plant species (28%) and the majority of frugivore species in the dataset (65%) were classified as endemic to the Caribbean. High degrees of endemism in local species communities are characteristic of island ecosystems (Kier et al., 2009; Paulay, 1994). In a review of plant–frugivore interactions on the Galapagos archipelago, Heleno et al. (2011) found similarly high proportions of endemic frugivores in the species pool (71%), underlining the importance of endemic frugivores for island communities. By contrast, only a few species in the dataset were classified as introduced to the Caribbean (16% plants and 8% frugivores), which was lower than other studies on island ecosystems. Notably on Hawai'i, the proportion of introduced seed disperser species ranged from 50% to 100% for plants and from 60% to 100% for birds (Vizentin-Bugoni et al., 2019). On the Galapagos, the proportion of introduced plants and frugivores was 28% and 23%, respectively (Heleno et al., 2011). However, on the Galapagos, all introduced frugivore species were mammals, whereas in our data, the vast majority of introduced species were birds (86%) and only two species (14%) were mammals (a primate: Chlorocebus pygerythrus and a carnivore: Herpestes javonicus).4.2 Cross-island patterns of shared species and interactionsWhen examining the role of different groups of plants and frugivores in connecting islands and archipelagos, we found that nonendemic native species and interaction pairs were shared most widely across islands (Figure 2), which is expected, as these species are widespread species occurring throughout the Caribbean and the Neotropical mainland. They are thus supposedly good dispersers, and their ranges often occur across multiple islands and cross-borders of archipelagos (Dalsgaard et al., 2014). Although endemic frugivores made up more than 60% of the frugivore species, generally they overlapped much less between islands compared to nonendemic natives, which only accounted for less than 30% of the frugivores in the data (Table 2). This pattern is not surprising, since the distributional ranges of endemic species are per definition confined within limited geographical areas (Kricher, 2011), many species being single-island endemics or occurring on few islands within each of the archipelagoes, that is the Lucayan archipelago, the Greater and Lesser Antilles (Dalsgaard et al., 2014). In the Caribbean, for instance, there is a high number of single-island endemic frugivorous birds, such as various species of parrots like the Saint Vincent Parrot Amazona guildingii (Birds Caribbean, 2021). Introduced plant species were shared widely across the Caribbean (Figure 2), which was expected, as most were agricultural and widely cultivated plants, reflecting that the Caribbean is historically heavily impacted by humans (Kemp et al., 2020; Walters & Hansen, 2013). By contrast, introduced frugivores were reported on few islands only (Table 2), and these islands shared mostly low proportions of introduced frugivores (Table S4); introduced interaction pairs were almost not shared between islands. Globally, a recent study showed how introduced species caused an increase in the proportion of regions sharing species and interactions (Fricke & Svenning, 2020), demonstrating that species introductions led to increasing similarity and homogenization in plant–frugivore communities across the world (Fricke & Svenning, 2020). In the Caribbean, however, given our data, especially nonendemic natives played a bigger role in interconnecting islands.4.3 Modularity of the Caribbean plant–frugivore meta-networkThe Caribbean plant–frugivore meta-network was organized in modules, as are most mutualistic plant–animal interaction networks, both local networks (e.g. Dalsgaard et al., 2013; Dupont & Olesen, 2009; Mello et al., 2011a, 2011b; Olesen et al., 2007) and meta-networks (Araujo et al., 2018; Emer et al., 2018; Martín González et al., 2018). The separation of the meta-network into modules was at least partly driven by functional or taxonomic (i.e. modules dominated by certain species groups) and biogeographical (i.e. island-specific modules) mechanisms. For instance, one module consisted of small- to medium-sized bird species recorded in Jamaica (100% birds; 88% of frugivores recorded in Jamaica; module nine in Figure 4). Another module consisted mostly of various bat species (63% bats) recorded in Cuba (88% of frugivores recorded in Cuba; module one in Figure 4), whereas another module consisted almost exclusively of rock iguanas (Cychlura spp.) found in the Bahamas only (88% Iguanas; 88% of frugivores were recorded on the Bahamas only; module 13 in Figure 4). These modules associated with specific functional/taxonomic groups or specific islands were thus positioned in the periphery of the Caribbean meta-network (Figure 3). The separation into modules according to biogeographical affinities, such as single islands, was expected given that interactions between plants and frugivores are inherently spatial as species must be in the same place to interact (Morales & Vázquez, 2008) and many species are restricted to specific islands. Spatial patterns that correspond to insularity in the broad sense have previously been shown to partially explain the modular structure of mutualistic plant–animal networks in landscape matrices, where species are restricted to different types of patchily distributed habitats (Maruyama et al., 2014). Patterns of modularity have also previously been suggested to be explained by behavioural or functional traits of species (Dicks et al., 2002; Donatti et al., 2011; Maruyama et al., 2014). In plant–frugivore interactions, although plants typically aim to attract functionally diverse seed dispersers (Plein et al., 2013), there is evidence of functional matching between interaction partners, especially with birds (Vollstädt et al., 2017). Morphologically different frugivore species tend to forage on morphologically distinct sets of plant species (Dehling et al., 2016; Gautier-Hion et al., 1985; Lomáscolo et al., 2010; Mello et al., 2011b), which might be reflected in the modules composed primarily of specific frugivore groups with characteristic morphological and functional traits. Bats, for instance, consume different types of fruits than birds and may show a clear separation in their dietary composition (Gorchov et al., 1995). The patterns of modularity we detected were therefore in line with expectations of functional/taxonomic and biogeographical mechanisms as drivers of modularity. However, there were also modules consisting of a mix of species from various islands. One module consisted of about 50% of large parrot species (Amazona spp.), but the frugivores were recorded in the entire Caribbean (module six in Figure 4) and, notably, the module in the centre of the Caribbean meta-network consisted of various types of frugivores occurring throughout the Caribbean, thereby interconnecting islands and archipelagos in the Caribbean meta-network (module seven; Figure 3 and Table S5).4.4 Interactions with introduced speciesRegarding how introduced species were integrated into the meta-network, we found that nonendemic native and endemic plants and frugivores interacted significantly less with introduced frugivore species than expected at random (Figure 4a,b). Among Caribbean frugivores and their fruiting plants, there is therefore no support for the idea that endemic super-generalists are the main facilitators of introduced species, as suggested for pollination networks on tropical islands (Olesen et al., 2002). On the contrary, introduced frugivores were recorded interacting with introduced plants significantly more often than expected at random (Figure 4c). This pattern suggests that introduced frugivores “prefer” to feed on introduced plants, which in turn suggests the presence of “invader complexes,” that is introduced species interacting more among themselves than expected at random, thus facilitating their establishment (D'Antonio & Dudley, 1993). Such facilitation processes between introduced species can lead to “invasional meltdowns,” as large groups of introduced species may have increasingly negative impacts on native communities (Jeschke et al., 2012; Simberloff & von Holle, 1999). Other island ecosystems have been found to be even more dominated by introduced frugivores, notably Hawai'i is almost exclusively dominated by introduced frugivores, as most of the endemic species have gone extinct (Vizentin-Bugoni et al., 2019; Vizentin-Bugoni et al., 2021). These findings from various archipelagos are concerning, regarding the potential impact of introduced species on native ecosystems. Such findings are particularly worrying when considering that on other island ecosystems, introduced species were also more often involved in seed-dispersal interactions (rather than seed/pulp predation) than native species (Heleno et al., 2011; Vizentin-Bugoni et al., 2019, 2021). For many of the interaction records, our data do not distinguish between seed-dispersal interactions or seed/pulp predation events; thus, it is not possible to estimate the effect of introduced species on local native and endemic plant communities in the Caribbean. Nevertheless, in Hawai'i, it was shown that introduced frugivores do not sufficiently replace the species roles of lost seed dispersers, since they preferentially disperse seeds of introduced rather than native plants (Vizentin-Bugoni et al., 2019). This raises the question why introduced plant species seem so attractive. One reason could be that introduced plants may have specific traits, such as longer fruiting duration, which increase the probability of encounters and are therefore more likely to be consumed by frugivores (Heleno et al., 2011; Sperry et al., 2021). In the Caribbean meta-network, many of the observations were from agricultural areas, where agricultural plants such as Mangifera indica (Mango) are often abundant with large crops, and although they are not dispersed by any native frugivore, they do overall attract many frugivores. Fruiting plant and thus resource abundance is in turn linked to increased fruit consumption, because frugivores often track available fruits in the landscape (Quitián et al., 2019), and consequently, the patterns we find may be partially driven by the high abundance of introduced agricultural plants and their crop sizes in human-dominated environments. Such patterns may be more pronounced on densely populated islands than on islands with few people and relatively more protected areas.In addition to “invader complexes,” we found that generalist species, that is species with many interaction partners, were more likely to interact with introduced species, which was consistent for both plants and frugivores (Figure S2a,b). These results are in line with previous findings, underlining the importance of highly generalized species for the establishment of introduced species, especially on islands (Maruyama et al., 2016). This gives support for the “preferential attachment” hypothesis (Newman, 2001), that is that species with wide ecological niches include and facilitate the establishment of new species, such as introduced species on islands. Our finding that generalized species do not have a higher proportion of interactions with introduced partners in their total set of interactions than specialized species (Figure S2c,d) reflects the overall low numbers of introduced species in the Caribbean data. Since only few of the potentially available interaction partners are introduced species, generalized species with many interaction partners would also be expected to have a decreasing proportion of their interactions with introduced species. Thus, although generalized species are likely to incorporate introduced species into their niche (Figure S2a,b), they do not have a specific preference for introduced species (Figure S2c,d).5 CONCLUSIONSBased on a comprehensive review of accessible data on plant–frugivore interactions, we showed that the Caribbean meta-network is structured into modules and demonstrate how introduced species are integrated into native communities in the Caribbean archipelago. These results provide valuable insight into plant–frugivore interactions in insular biodiversity hotspots, showing how insular plant–frugivore systems are susceptible to invasion. Future studies are needed to demonstrate the importance of introduced species as seed dispersers compared with seed/pulp predators (Nogales et al., 2017). Specifically, research quantifying the relative importance of different frugivore groups as seed dispersers and their respective effectiveness is lacking for most plant–frugivore interactions in the Caribbean. This would provide valuable information and could help with the conservation of endemic plants in the Caribbean archipelago. Moreover, we also in general lack information on frugivory in the Caribbean. Kim et al. (2022) reported 4336 species of plants with animal-dispersal syndromes in the Caribbean archipelago, and our dataset represents only 11% of those species with some regional variation (Table S6). For instance, whereas Puerto Rican plants were covered relatively well (31% of the species), plants in Hispaniola (approx. 7%), Jamaica (approx. 8%) and the Lesser Antilles (approx. 9%) were less well represented. There may also be taxonomical differences in sampling completeness. Palms (Arecaceae) are highly diverse in the Caribbean representing 135 species (Roncal et al., 2008), and our dataset had only 23 palm species (17%). Several endemic and highly threatened fleshy-fruited plants do not have any information on the main seed dispersers (e.g. Catesbea spinosa, Brunfelsia portoricensis, Diospyros spp. and many cactus species). We also have limited and incomplete information on the fruit diet of several endemic frugivores (e.g. pigeons, thrashers and thrushes) that could play an important role for seed dispersal of Caribbean plants. There is therefore an urgent need to increment more scientific information on plant–frugivore interactions in the Caribbean, one of the world's insular biodiversity hotspots.ACKNOWLEDGEMENTSThis work is dedicated to our colleague and coauthor Michel Faife-Cabrera who passed away due to Covid-19 complications. M. Galetti thanks CNPq and University of Miami for financial support. M.G.R. Vollstädt, C. N. Kaiser-Bunbury, B. I. Simmons, F. Gonçalves, and B. Dalsgaard thank the Independent Research Fund Denmark (grant no. 0135-00333B). Funding for A. Strong's work was provided by an NSF grant to T. W. Sherry (Tulane University) and R. T. Holmes (Dartmouth College), the Chicago Zoological Society, Sigma Xi Grants-in-Aid-of Research, the World Nature Association, and The Louisiana Educational Quality Support Fund. A. Strong's work benefitted from collaborations with M. Johnson, T. Sherry, A. Sutton and the late R. Sutton. Funding for S. Schubert's work was provided by Rufford Foundation Small Grants 1, 2, & Booster, in addition to the Old Dominion University Paul W. Kirk Jr Student Research Award, a British Ornithologists' Union Student Research Award, and the BirdsCaribbean David S. Lee Fund. J. Salazar's work was funded by FONDOCyT. (Ministerio de Educaciòn Superior, Ciencia y Tecnología), Project 1B4-9. F. L. Tarazona-Tubens is supported by McKight Fellowship. B. I. Simmons was supported by a Royal Commission for the Exhibition of 1851 Research Fellowship. We also thank all researchers who have worked intensively in the Caribbean.CONFLICT OF INTERESTThe authors declare that there is no conflict of interest to report.Open ResearchSupporting InformationREFERENCESBIOSKETCHMaximilian Vollstädt is an ecologist, and a researcher at the University of Copenhagen, Denmark. His research interests include mutualistic interactions in plant-pollinator and plant-seed disperser communities in tropical island ecosystems.Author contributions: M.G.R.V., M.G., B.D. Conceptualization, Methodology; M.G.R.V., B.I.S. Formal analysis, Visualization; M.G.R.V., M.G., C.N.K.B., B.D. Original draft preparation, Writing; All Authors: Writing, Review & Editing.Volume28, Issue11November 2022Pages 2361-2374FiguresReferencesRelatedInformationRecommendedNatural mixing of species: novel plant–animal communities on Caribbean IslandsA. E. Lugo, T. A. Carlo, J. M. WunderleAnimal ConservationFrugivore distributions are associated with plant dispersal syndrome diversity in the Caribbean archipelagosSeokmin Kim, Lilian Sales, Daiane Carreira, Mauro GalettiDiversity and DistributionsInteractions between resource availability and enemy release in plant invasionDana M. BlumenthalEcology LettersHuman disturbances affect the topology of food websFrederico Mestre, Alejandro Rozenfeld, Miguel B. AraújoEcology LettersA general framework for species-abundance distributions: Linking traits and dispersal to explain commonness and rarityThomas Koffel, Kaito Umemura, Elena Litchman, Christopher A. Klausmeier
Flemming Javier Olsen; Mats Christian Højbjerg Lassen; Kristoffer Grundtvig Skaarup; Jacob Christensen; Filip Soeskov Davidovski; Alia Saed Alhakak; Morten Sengeløv; Anne Bjerg Nielsen; Niklas Dyrby Johansen; Claus Graff; +23 more
Flemming Javier Olsen; Mats Christian Højbjerg Lassen; Kristoffer Grundtvig Skaarup; Jacob Christensen; Filip Soeskov Davidovski; Alia Saed Alhakak; Morten Sengeløv; Anne Bjerg Nielsen; Niklas Dyrby Johansen; Claus Graff; Henning Bundgaard; Christian Hassager; Reza Jabbari; Jørn Carlsen; Ole Kirk; Matias Greve Lindholm; Lothar Wiese; Ole Peter Kristiansen; Olav W. Nielsen; Birgitte Lindegaard; Niels Tønder; Charlotte Suppli Ulrik; Morten Lamberts; Pradeesh Sivapalan; Gunnar Gislason; Kasper Iversen; Jens Ulrik Stæhr Jensen; Morten Schou; Jesper Hastrup Svendsen; John Moene Aalen; Otto Armin Smiseth; Espen Wattenberg Remme; Tor Biering‐Sørensen;
Background COVID‐19 infection has been hypothesized to affect left ventricular function; however, the underlying mechanisms and the association to clinical outcome are not understood. The global work index (GWI) is a novel echocardiographic measure of systolic function that may offer insights on cardiac dysfunction in COVID‐19. We hypothesized that GWI was associated with disease severity and all‐cause death in patients with COVID‐19. Methods and Results In a multicenter study of patients admitted with COVID‐19 (n=305), 249 underwent pressure‐strain loop analyses to quantify GWI at a median time of 4 days after admission. We examined the association of GWI to cardiac biomarkers (troponin and NT‐proBNP [N‐terminal pro‐B‐type natriuretic peptide]), disease severity (oxygen requirement and CRP [C‐reactive protein]), and all‐cause death. Patients with elevated troponin (n=71) exhibited significantly reduced GWI (1508 versus 1707 mm Hg%; P =0.018). A curvilinear association to NT‐proBNP was observed, with increasing NT‐proBNP once GWI decreased below 1446 mm Hg%. Moreover, GWI was significantly associated with a higher oxygen requirement (relative increase of 6% per 100–mm Hg% decrease). No association was observed with CRP. Of the 249 patients, 37 died during follow‐up (median, 58 days). In multivariable Cox regression, GWI was associated with all‐cause death (hazard ratio, 1.08 [95% CI, 1.01–1.15], per 100–mm Hg% decrease), but did not increase C‐statistics when added to clinical parameters. Conclusions In patients admitted with COVID‐19, our findings indicate that NT‐proBNP and troponin may be associated with lower GWI, whereas CRP is not. GWI was independently associated with all‐cause death, but did not provide prognostic information beyond readily available clinical parameters. Registration URL: https://www.clinicaltrials.gov ; Unique identifier: NCT04377035.
Nora Fagerholm; Karl Samuelsson; Salla Eilola; Matteo Giusti; Kamyar Hasanzadeh; Anna Kajosaari; Daniel Koch; Silviya Korpilo; Marketta Kyttä; Ann Legeby; +6 more
Nora Fagerholm; Karl Samuelsson; Salla Eilola; Matteo Giusti; Kamyar Hasanzadeh; Anna Kajosaari; Daniel Koch; Silviya Korpilo; Marketta Kyttä; Ann Legeby; Yu Liu; Søren Præstholm; Christopher Raymond; Tiina Rinne; Anton Stahl Olafsson; Stephan Barthel;
Publisher: Springer Science and Business Media LLC
Countries: Finland, Denmark, Sweden
Project: AKA | Wellbeing benefits of urb... (321555)
AbstractRecent empirical research has confirmed the importance of green infrastructure and outdoor recreation to urban people’s well-being during the COVID-19 pandemic. However, only a few studies provide cross-city analyses. We analyse outdoor recreation behaviour across four Nordic cities ranging from metropolitan areas to a middle-sized city. We collected map-based survey data from residents (n = 469–4992) in spring 2020 and spatially analyse green infrastructure near mapped outdoor recreation sites and respondents’ places of residence. Our statistical examination reveals how the interplay among access to green infrastructure across cities and at respondents’ residential location, together with respondents’ socio-demographic profiles and lockdown policies or pandemic restrictions, affects outdoor recreation behaviour. The results highlight that for pandemic resilience, the history of Nordic spatial planning is important. To support well-being in exceptional situations as well as in the long term, green infrastructure planning should prioritise nature wedges in and close to cities and support small-scale green infrastructure.
Christian Erikstrup; Anna Damkjær Laksafoss; Josephine Gladov; Kathrine Agergård Kaspersen; Susan Mikkelsen; Lotte Hindhede; Jens Kjærgaard Boldsen; Signe Winther Jørgensen; Steen Ethelberg; Dorte Kinggaard Holm; +17 more
Christian Erikstrup; Anna Damkjær Laksafoss; Josephine Gladov; Kathrine Agergård Kaspersen; Susan Mikkelsen; Lotte Hindhede; Jens Kjærgaard Boldsen; Signe Winther Jørgensen; Steen Ethelberg; Dorte Kinggaard Holm; Mie Topholm Bruun; Janna Nissen; Michael Schwinn; Thorsten Brodersen; Christina Mikkelsen; Susanne Gjørup Sækmose; Erik Sørensen; Lene Holm Harritshøj; Bitten Aagaard; Khoa Manh Dinh; Michael P. Busch; Charlotte Sværke Jørgensen; Tyra Grove Krause; Henrik Ullum; Sisse Rye Ostrowski; Laura Espenhain; Ole Birger Vesterager Pedersen;
Background: Introduction of the Omicron variant caused a steep rise in SARS-CoV-2 infections despite high vaccination coverage in the Danish population. We used blood donor serosurveillance to estimate the percentage of recently infected residents in the similarly aged background population with no known comorbidity.Methods: To detect SARS-CoV-2 antibodies induced due to recent infection, and not vaccination, we assessed anti-nucleocapsid (anti-N) immunoglobulin G (IgG) in blood donor samples. Individual level data on SARS-CoV-2 RT-PCR results and vaccination status were available. Anti-N IgG was measured fortnightly from January 18 to April 3, 2022. Samples from November 2021 were analysed to assess seroprevalence before introduction of the Omicron variant in Denmark.Findings: A total of 43 088 donations from 35 309 Danish blood donors aged 17-72 years were screened. In November 2021, 1·2% (103/8 701) of donors had detectable anti-N IgG antibodies. Adjusting for test sensitivity (estimates ranging from 74%-81%) and November seroprevalence, we estimate that 66% (95% confidence intervals (CI): 63%-70%) of the healthy, similarly aged Danish population had been infected between November 1, 2021, and March 15, 2022. One third of infections were not captured by SARS-CoV-2 RT-PCR testing. The infection fatality rate (IFR) was 6·2 (CI: 5·1-7·5) per 100 000 infections.Interpretation: Screening for anti-N IgG and linkage to national registers allowed us to detect recent infections and accurately assess assay sensitivity in vaccinated or previously infected individuals during the Omicron outbreak. The IFR was lower than during previous waves.Funding: The Danish Ministry of Health.
Dennis Christensen; Charlotta Polacek; Daniel J. Sheward; Leo Hanke; Ainhoa Moliner-Morro; Gerald McInerney; Ben Murrell; Katrine Top Hartmann; Henrik Elvang Jensen; Gregers Jungersen; +10 more
Dennis Christensen; Charlotta Polacek; Daniel J. Sheward; Leo Hanke; Ainhoa Moliner-Morro; Gerald McInerney; Ben Murrell; Katrine Top Hartmann; Henrik Elvang Jensen; Gregers Jungersen; Kristin Illigen; Louise Krag Isling; Rune Fledelius Jensen; Julia Sid Hansen; Ida Rosenkrands; Carlota Fernandez-Antunez; Santseharay Ramirez; Frank Follmann; Jens Bukh; Gabriel Kristian Pedersen;
BackgroundLicensed vaccines against SARS-CoV-2 effectively protect against severe disease, but display incomplete protection against virus transmission. Mucosal vaccines providing immune responses in the upper airways are one strategy to protect against transmission.MethodsWe administered Spike HexaPro trimer formulated in a cationic liposomal adjuvant as a parenteral (subcutaneous – s.c.) prime - intranasal boost regimen to elicit airway mucosal immune responses and evaluated this in a Syrian hamster model of virus transmission.FindingsParenteral prime - intranasal boost elicited high-magnitude serum neutralizing antibody responses and IgA responses in the upper respiratory tract. The vaccine strategy protected against virus in the lower airways and lung pathology, but virus could still be detected in the upper airways. Despite this, the parenteral prime - intranasal booster vaccine effectively protected against onward SARS-CoV-2 transmission.InterpretationThis study suggests that parenteral-prime mucosal boost is an effective strategy for protecting against SARS-CoV-2 infection and highlights that protection against virus transmission may be obtained despite incomplete clearance of virus from the upper respiratory tract. It should be noted that protection against onward transmission was not compared to standard parenteral prime-boost, which should be a focus for future studies.FundingThis work was primarily supported by the European Union Horizon 2020 research and innovation program under grant agreement no. 101003653.
Purpose: Cystoscopy procedures can cause distress among patients. Patient perspectives on health services are essential inputs in decision-making. This study investigated the patient preferences in Spain regarding single-use cystoscopes (SUC) compared to reusable cystoscopes and their willingness to pay (WTP) for cystoscopy procedures.Patients and Methods: Between May and June 2021, an anonymous survey was distributed to Spanish patients who had previously undergone a cystoscopy. The survey included patient preference measures on reusable cystoscopes compared to SUCs and a discrete choice experiment. The survey was distributed through a human data science company (IQVIA), collected using an online survey tool (QuestionPro (R)), and analyzed using Stata/MP, StataCorp.Results: Of 300 respondents, 148 (49.33%) were female and 150 (50%) were male, and mainly between 18-49 years (247, 82.33%). Most (265, 88%) preferred to have their procedure performed with a SUC rather than a reusable cystoscope. Among these patients, 215 (80%) could imagine asking their doctor to use a SUC. A total of 231 (77%) respondents indicated an increased level of concern about the risk of exposure to contamination related to their cystoscopy following the COVID-19 pandemic. Patients would pay 62 EUR to have their initial consultation and cystoscopy procedure on the same day (p < 0.001), 59 EUR to reduce the environmental impact, and 57 EUR to reduce the risk of contamination (p < 0.001).Conclusion: Patients prefer to undergo cystoscopy using an SUC on the same day as their initial consultation. The increased contamination concerns due to the COVID-19 pandemic and WTP to reduce the risk of cystoscope contamination may explain patients' preferences for SUCs. The most important attributes related to their cystoscopy procedure are the ability to have their procedure performed on the same day as their initial consultation, the reduction of the environmental impact, and the reduction of the contamination risk.
This study aimed to investigate the potential protective role of baseline resources and capabilities for experiencing challenges to emotional well-being and perceived access to and quality of diabetes care during the COVID-19 pandemic in a Danish type 2 diabetes population (N = 1608). We investigated how differences in self-efficacy, well-being capability, socioeconomic status, health status, and perceptions of diabetes care measured before the COVID-19 pandemic were related to experiences of well-being and diabetes management challenges during the pandemic. The study is based on a survey conducted shortly before the pandemic (autumn 2019) and a follow-up survey during the pandemic (autumn 2020), which included questions about impacts of the pandemic. We used this longitudinal data to quantitatively investigate in regression analyses how self-reported baseline indicators of chronic care access and quality (PACIC), self-efficacy (GSE), health (EQ VAS), and well-being capability (ICE-CAP-A), and registry-based socioeconomic indicators were associated with the probability of reporting negative impacts on emotional wellbeing and diabetes management. Results showed that respondents with higher baseline general self-efficacy and higher well-being capability scores, who more often considered care well-organised and were in better health before the pandemic, were less likely to report pandemic-related negative impacts on emotional well-being. Considering diabetes care well organised before the pandemic was associated with a lower probability of adverse impacts on diabetes care. The results thus broadly confirmed that several indicators of higher levels of baseline resources and capabilities were associated with a lower probability of reporting negative impacts of the pandemic. However, some variation in predictors was observed for general well-being outcomes, compared to diabetes-care specific challenges, and findings on socioeconomic status as indicated by education were mixed.