Advanced search in Research products
Research products
arrow_drop_down
Searching FieldsTerms
Subject
arrow_drop_down
includes
arrow_drop_down
Include:
The following results are related to COVID-19. Are you interested to view more results? Visit OpenAIRE - Explore.
1,625 Research products, page 1 of 163

  • COVID-19
  • Conference object

10
arrow_drop_down
Relevance
arrow_drop_down
  • Publication . Conference object . 2021
    Open Access English
    Authors: 
    Shaheen, Ibraheem Theeb; Kurdi, Yahya Nazem;
    Publisher: Maltepe Üniversitesi
    Country: Turkey

    The Coronavirus or SARS-CoV-2, is a highly pathogenic β- coronavirus that infects humans through air transmission remotely. The most critical step in Early Diagnosis is to treat the infection. The Diagnostic methods include nasal swab, rectal swab recently used in China, Bronchio-alveolar leavage, chest x-ray, CT-san, Ultrasound, viral culture, serology, and molecular methods. CRISPR-based method is used to diagnose and treat the infection. Presence of IgG and IgM in blood examination [1]. Also a viral Gene detection, human antibody detection and viral antigen, amidst the viral gene detection by the RT-PCR is the most a ested technique [2]. Antiviral Inhibitors of the spike proteins of SARS and MERS, favipiravir, Azithromycin, antipyretics, α- interferon, anticoagulants, anti-inflammatory drugs including corticosteroids and interleukins inhibitors, neuraminidase inhibitors and EK1 peptide are the therapeutic ways for SARS-CoV-2 infection. Hydroxychloroquine which was the treatment method for malaria has shown results in the SARS-CoV-2 treatment. Using and oxygen support can assuage the symptoms effectively [1]. A research was performed on the observation techniques and quantitative computable conviction methods of chloroquine and its related metabolites. Azithromycin is given with Hydroxychloroquine as a combination for treatment and anticoagulants for prevention of further complications [3]. The drugs hydroxychloroquine and chloroquine have antiviral characteristics immunologically in vitro. These findings show that these drugs have potency in treatment. But they have serious side effects, ranging from psychiatric to sudden death. So, favipiravir is the best drug of choice currently along with anticoagulants to prevent thrombosis [3][4].

  • Open Access Serbian
    Authors: 
    Jelesijević, Tomislav; Aleksić-Kovačević, Sanja;
    Publisher: Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
    Country: Serbia

    U novembru 2002. godine, u kineskoj provinciji Guandong poče- la je epidemija, životno ugrožavajuće respiratorne bolesti nepoznate etiologije. Bolest se odlikovala teškim akutnim respiratornim sindro- mom i skraćeno se naziva SARS, a pred kraj ove pandemije, otkrive- no je da je prouzrokovana novim korona virusom (SARS-CoV). Iako su azijske cibetke (Pagumalarvata) direktno prenosile prepandemij- ski soj virusa na ljude, stvarni izvori virusa bile su različite vrste slepih miševa. Bolest se iz Kine proširila na 30 zemalja, dijagnostikovana je kod 8 000 ljudi i prouzrokovala je stopu smrtnosti od 10 posto. Slično prvoj, druga pandemija SARS-a je počela u kineskom gradu Vuhanu 2019. Godine i bila je povezana sa najmanje dve vrste sisara: pango- linima i slepim miševima. SARS-CoV 2, kao i bolest koja iz njega proi- stiče - COVID 19, su ovoga puta zarazili desetine miliona ljudi, godinu dana od izbijanja pandemije. COVID 19 je prouzrokovao stopu smrt- nosti od 2,4% i značajno je uticao na zdravstvene, obrazovne i eko- nomske sisteme i živote ljudi. Pokrenuta su brojna komparativna ispiti- vanja na animalnim modelima i uočene su razlike, ali i brojne sličnosti koronavirusnih infekcija mačaka i ljudi. Ponovljeni način prenošrnja i procena postojanja nekoliko hiljada korona virusa slepih miševa su- gerišu da prošla, sadašnja i buduća pandemija SARS-a, ne treba da budu iznenađenje.

  • Open Access English
    Authors: 
    Barbara Colitti; Francesco Bonfante; Santina Grazioli; Laura Anfossi; Chiara Nogarol; Daniela Scalas; Luigi Bertolotti; Alessio Bortolami; Matteo Pagliari; Giulia Pezzoni; +6 more
    Publisher: ESCMID
    Country: Italy

    Since the initial emergence in December 2019, the novel Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has been reported in over 200 countries, representing an unprecedented challenge related to disease control worldwide. In this context, cases of human to animal transmission have been reported, raising concern about the potential role of companion animals in the pandemic and stressing the need for reliable animal testing. In the study, a detailed epitope mapping of SARS-CoV-2 nucleoprotein, using both human and pet sera, allowed the identification of the most antigenic region in the C-terminus domain of the protein, which was used to develop an experimental double antigen-based ELISA. A panel of pre-pandemic sera and sera of animals immunized against (or naturally infected with) related coronaviruses was used to assess assay specificity at 99.5%. Positive sera belonging to animals housed with COVID-19 patients were confirmed with the experimental double-antigen ELISA using Plaque Reduction Neutralization test (PRNT) test as gold standard. The availability of a serological assay that targets a highly specific viral antigen represents a valuable tool for multispecies monitoring of Coronavirus Disease 2019 (COVID-19) infection in susceptible animals.

  • Restricted Danish
    Authors: 
    Dessau, Ram;
    Country: Denmark
  • Russian
    Publisher: Федеральное государственное бюджетное учреждение науки Центральный экономико-математический институт Российской академии наук
  • Publication . Other literature type . Conference object . 2022
    Open Access Portuguese
    Authors: 
    Cristina Mesquita; Ana Claudia Loureiro; Cristiana Ribeiro;
    Publisher: Universidade Politècnica de València
    Countries: Portugal, Portugal, Spain

    Alguns estudos têm revelado que a pandemia COVID-19 tem consequências devastadoras a curto, médio e longo prazo para as crianças e que se constitui como um fator inibidor dos seus direitos. Tem sido evidenciado, que pode ter graves efeitos a nível físico, emocional e psicológico nas crianças, especialmente em países que tomaram medidas de permanência em casa, obrigando ao confinamento das crianças, condicionando o seu contacto com as outras crianças e, em muitos casos com o ambiente natural. Este estudo pretende-se identificar as conceções que as crianças têm sobre o COVID-19 e a forma como têm vivenciado estes tempos de confinamento físico e a alteração da sua vida social. Para aceder às perceções das crianças, realizou-se um estudo de natureza qualitativa, utilizando a entrevista semiestruturada e o desenho livre como instrumentos de recolha de dados. Os participantes no estudo foram 135 crianças, com idades compreendidas entre os 3 e os 5 anos de idade, de jardins de infância da rede pública, rural e urbana. Todos os procedimentos éticos foram considerados, garantindo o anonimato, a confidencialidade e o consentimento informado das crianças, educadores e famílias ou instituição. As entrevistas às crianças foram registadas e, posteriormente, submetidas à análise de conteúdos da qual surgiram categorias que permitiram a análise dos discursos. Os resultados obtidos salientam que as crianças possuem a noção realista sobre o vírus e os seus efeitos na saúde das pessoas e das suas consequências para a sociedade. Reconhecem também que as medidas tomadas foram necessárias para controlar a disseminação da doença, mas expressam tristeza ao narrarem sobre a falta que sentiram de seus entes queridos, de seus colegas, de brincar fora de casa e de fazer comemorações. info:eu-repo/semantics/publishedVersion

  • Closed Access
    Authors: 
    Salomoni, Maria Giulia; Angelo, Capodici; Marco Montalti; Davide Gori;
    Country: Italy
  • Publication . Conference object . 2020
    English
    Authors: 
    Demyashkin, G.A.; Boldyrev, D.V.; Shchekin, V.I.;
    Publisher: Инфинити

    Summary Despite some progress in the study of the effect of COVID-19 infection on the human body, many issues related to pathological processes, as well as the developing deficiencies, remain not clear understood. One out of such issues is the effect of SARS-CoV-2 on fertility. The aim of this work is to assess the molecular profile in testicular tissue in patients affected by COVID-19. Materials and methods. Fragments of the testes of patients with confirmed COVID-19 (n = 48) were studied by real-time polymerase chain reaction to determine the expression of viral RNA. Results. in patients affected by COVID-19, the presence of coronavirus genetic material in the testis was recorded. Conclusion. Current research demonstrated the presence of SARS- CoV-2 in testicular tissue.

  • Open Access English
    Authors: 
    Simović, Ana; Radomirović, Mirjana Ž.; Gligorijević, Nikola; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja;
    Publisher: Sociedade Portuguesa de Química
    Country: Serbia
    Project: EC | FoodEnTwin (810752)

    Algae have been consumed as food and medicine for centuries. Their benefits are so pronounced, due to high concentrations of vitamins, minerals, antioxidants and proteins that they are commonly referred to as superfoods. Phycocyanobilin (PCB) is a bioactive compound of microalga Spirulina platensis. It is a blue tetrapyrrole chromophore of C-phycocyanin (C-PC), the major chromoprotein of this microalga. It is covalently attached to cysteine residues of C-PC via thioether bond. The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike (S) protein plays the most important roles in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. It mediates viral entry into host cells by first binding to a host angiotensin-converting enzyme 2 (ACE2) receptor through the receptor-binding domain (RBD) and then fusing the viral and host membranes. This study aimed to investigate interaction of bioactive PCB with S protein and RBD respectively. Combination of electrophoretic techniques and fluorescence spectroscopy was employed in order to examine interactions of PCB and S protein, as well as interactions of PCB and RBD, while the effects of PCB binding on RBD structure were studied by CD spectroscopy. SDS-PAGE with Zn2+ staining has revealed that PCB covalently binds to both S protein and RBD, via free cysteine residues. Binding constants determined by fluorescence quenching method were: 2.1 x 107 M–1 for PCB and S protein, and 8.4 x 104 M–1 for PCB and RBD. Far-UV circular dichroism spectra showed that PCB influences RBD structure. Our results support the importance of further research on covalent binding of PCB to S protein and RBD and its implications. Due to its interaction with S protein and RBD, PCB may exert one of its many bioactive effects via impact on S protein binding to ACE2 receptor. Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810752. References: [1] S. Minic, M. Radomirovic, N. Savkovic et al., Food Chemistry, 269(2018) 43-52. [2] W. Tai, L. He, X. Zhang et al., Cellular & Molecular Immunology, 17(2020) 613-620.

  • Open Access Turkish
    Authors: 
    Akman, Nazife; Akidağı, Zeynep; Özmen, Pelin; Yalap, Rukiye;
    Country: Turkey

    ÖZET Koronavirüs hastalığı 2019 (COVID-19) hızlı yayılım gösteren bir pandemidir. Hastalığın yayılmasında virüse bağlı etmenlerin (viral yük, ACE bağlama proteini vs.) yanı sıra asemptomatik bireylerin de rolü azımsanmayacak miktardadır. COVID-19 pandemisinde, toplumda virüsün yayılmasına neden olan sessiz bulaştırıcıları saptamak için Kapadokya Üniversitesi sağlık programlarında öğrenim gören 18-40 yaş arasındaki 258 öğrencide SARS-CoV-2 seroprevalansı araştırıldı. Katılımcıların yaş ortalaması 21,5 (min = 18, max= 33) olup %69’u (n=38) kız öğrenciydi. Çalışmada bireylerin seroprevalans taramasında LFIA (Lateral Flow Immuno Assay) yöntemi kullanılarak SARS-COV-2 virüsüne karşı antikor yanıtları tarandı ve hastalık bulguları, PZR (polimeraz zincir reaksiyonu) testi ve şüpheli temas öyküsü yönünden değerlendirildi. Çalışmamızda SARS-CoV-2 seropozitifliği %21 (n=55) olarak saptanmış olup bu bireylerin %25’inin (n=14) son altı ay içinde PZR pozitiflikleri bulunmaktaydı. %56’sının (n=31) semptom göstermediği için test yaptırmadığını fakat SARS-COV-2 antikor pozitifliği saptandı. Bu oranlar, toplumdaki sessiz bulaştırıcıların yaygınlığını ortaya koymuştur. Bu çalışma seroepidemiyolojik veri eldesi açısından değerli olmakla birlikte koruyucu bağışıklık göstergesi olarak kabul edilmemelidir.